Product Info Summary
SKU: | PA1477 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-FGFR1 Antibody
SKU/Catalog Number
PA1477
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FGFR1 Antibody catalog # PA1477. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FGFR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1477)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human FGFR1, different from the related mouse sequence by two amino acids and rat sequence by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1477 is reactive to FGFR1 in Human, Mouse, Rat
Applications
PA1477 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
24 kDa
Calculated molecular weight
91.868kDa
Background of FGFR1
FGFR1, Fibroblast growth factor receptor 1, also known as basic fibroblast growth factor receptor 1, fms-related tyrosine kinase-2/Pfeiffer syndrome, and CD331, is a receptor tyrosine kinase whose ligands are specific members of the fibroblast growth factor family. The FGFR1 gene is localized to 8p12-p11.2 by in situ hybridization. FGFR1 is essential for the normal formation of the organ of Corti and that phenotype severity observed in FGFR1 mutants is dependent on the dose of FGFR1.Mutations in this gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer and autosomal dominant Kallmann syndrome 2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-FGFR1 antibody, PA1477, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: SMMC Cell Lysate
Lane 4: HT1080 Cell Lysate
Lane 5: MM231 Cell Lysate
Click image to see more details
Anti-FGFR1 antibody, PA1477, IHC(P)
IHC(P): Human Lung Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For FGFR1 (Source: Uniprot.org, NCBI)
Gene Name
FGFR1
Full Name
Fibroblast growth factor receptor 1
Weight
91.868kDa
Superfamily
protein kinase superfamily
Alternative Names
basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; CD331 antigen; CD331; CEK; EC 2.7.10; EC 2.7.10.1; FGF R1; FGFBR; FGFR1; FGFR-1; fibroblast growth factor receptor 1; FLGH3; FLJ99988; Flt-2; FLT2H4; Fms-like tyrosine kinase 2; fms-related tyrosine kinase 2; H2; H5; HBGFR; heparin-binding growth factor receptor; hydroxyaryl-protein kinase; KAL 2; KAL2; N-SAM; OGD; Proto-oncogene c-Fgr; soluble FGFR1 variant 1; soluble FGFR1 variant 2 FGFR1 BFGFR, CD331, CEK, ECCL, FGFBR, FGFR-1, FLG, FLT-2, FLT2, HBGFR, HH2, HRTFDS, KAL2, N-SAM, OGD, bFGF-R-1 fibroblast growth factor receptor 1 fibroblast growth factor receptor 1|FGFR1/PLAG1 fusion|FMS-like tyrosine kinase 2|basic fibroblast growth factor receptor 1|fms-related tyrosine kinase 2|heparin-binding growth factor receptor|hydroxyaryl-protein kinase|proto-oncogene c-Fgr
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FGFR1, check out the FGFR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FGFR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FGFR1 Antibody (PA1477)
Hello CJ!
PA1477 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang JJ,Zhou XH,Zhou Y,Wang YG,Qian BZ,He AN,Shen Z,Hu HY,Yao Y.Bufalin suppresses the migration and invasion of prostate cancer cells through HOTAIR, the sponge of miR-520b.Acta Pharmacol Sin.2019 Sep;40(9):1228-1236.doi:10.1038/s41401-019-0234-8.Epub 2019 Apr 26.PMID:31028291;PMCID:PMC6786369.
Species: Human,Rat
PA1477 usage in article: APP:IHC, SAMPLE:PROSTATE TISSUE, DILUTION:NA
Chen Y,Zhang S,Bai C,Guan Z,Chen W.Circ_0000885 Enhances Osteosarcoma Progression by Increasing FGFR1 Expression via Sponging MiR-1294.Cancer Manag Res.2020 Jul 28;12:6441-6452.doi:10.2147/CMAR. S244382.PMID:32801884;PMCID:PMC7395693.
Species: Human, Mouse
PA1477 usage in article: APP:WB, SAMPLE:SAOS-2 CELLS AND SOSP-9607 CELLS, DILUTION:1:400
Regulation of 25-hydroxyvitamin D-1-hydroxylase and 24-hydroxylase in keratinocytes by PTH and FGF23
Zhang H, Zhang S, Zhuang H, Lu F. Cancer Biother Radiopharm. 2006 Aug;21(4):321-32. Cytotoxicity Of A Novel Fibroblast Growth Factor Receptor Targeted Immunotoxin On A Human Ovarian Teratocarcinoma Cell Line.
Lin J, Jia Y, Zeng W, Mi Y, Zhang C. Reprod Domest Anim. 2012 Feb;47(1):135-42. Doi: 10.1111/J.1439-0531.2011.01813.X. Epub 2011 May 26. Basic Fgf Promotes Proliferation Of Ovarian Granulosa Cells In The Laying Chickens Via Fgfr1 And Pkc Pathway.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-FGFR1 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-FGFR1 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-FGFR1 Antibody
Question
Can PA1477 work well on mouse brain sample?
Verified customer
Asked: 2019-12-11
Answer
The Anti-FGFR1 Antibody PA1477 works well on mouse brain sample. here is a link to access our IHC protocols, https://www.bosterbio.com/protocol-and-troubleshooting/ihc-protocol
Boster Scientific Support
Answered: 2019-12-11
Question
My lab would like using your anti-FGFR1 antibody for positive regulation of mitotic cell cycle dna replication studies. Has this antibody been tested with western blotting on lung cancer tissue? We would like to see some validation images before ordering.
T. Dhar
Verified customer
Asked: 2019-04-16
Answer
We appreciate your inquiry. This PA1477 anti-FGFR1 antibody is tested on rat liver tissue, tissue lysate, brain tissue, smmc cell lysate, ht1080 cell lysate, mm231 cell lysate, lung cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-04-16
Question
We are currently using anti-FGFR1 antibody PA1477 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-20
Answer
The anti-FGFR1 antibody (PA1477) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-20
Question
We have been able to see staining in mouse liver. Any tips? Is anti-FGFR1 antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2018-10-16
Answer
Based on literature liver does express FGFR1. Based on Uniprot.org, FGFR1 is expressed in body of pancreas, placenta, neonatal brain stem, teratocarcinoma, lung, liver, placenta testis, brain colon, pancreas, testis uterus, foreskin fibroblast umbilical vein, plasma, among other tissues. Regarding which tissues have FGFR1 expression, here are a few articles citing expression in various tissues:
Brain, and Colon, Pubmed ID: 16421571
Foreskin fibroblast, and Umbilical vein, Pubmed ID: 1652059
Liver, Pubmed ID: 1846977
Lung, Pubmed ID: 1650441
Neonatal brain stem, Pubmed ID: 1697263
Pancreas, Testis, and Uterus, Pubmed ID: 15489334
Placenta, Pubmed ID: 2159626, 2162671
Placenta, and Testis, Pubmed ID: 14702039
Plasma, Pubmed ID: 16335952
Teratocarcinoma, Pubmed ID: 1722683
Boster Scientific Support
Answered: 2018-10-16
Question
Our lab were satisfied with the WB result of your anti-FGFR1 antibody. However we have seen positive staining in placenta testis cell membrane using this antibody. Is that expected? Could you tell me where is FGFR1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-07-13
Answer
According to literature, placenta testis does express FGFR1. Generally FGFR1 expresses in cell membrane. Regarding which tissues have FGFR1 expression, here are a few articles citing expression in various tissues:
Brain, and Colon, Pubmed ID: 16421571
Foreskin fibroblast, and Umbilical vein, Pubmed ID: 1652059
Liver, Pubmed ID: 1846977
Lung, Pubmed ID: 1650441
Neonatal brain stem, Pubmed ID: 1697263
Pancreas, Testis, and Uterus, Pubmed ID: 15489334
Placenta, Pubmed ID: 2159626, 2162671
Placenta, and Testis, Pubmed ID: 14702039
Plasma, Pubmed ID: 16335952
Teratocarcinoma, Pubmed ID: 1722683
Boster Scientific Support
Answered: 2017-07-13
Question
We purchased anti-FGFR1 antibody for IHC on body of pancreas a few years ago. I am using human, and We are going to use the antibody for WB next. I am looking for examining body of pancreas as well as placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
N. Brown
Verified customer
Asked: 2013-02-19
Answer
I have checked the website and datasheets of our anti-FGFR1 antibody and it seems that PA1477 has been tested on human in both IHC and WB. Thus PA1477 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-02-19