Anti-FGR Antibody Picoband™

Product Name

Anti-FGR Antibody Picoband™

View all FGR Antibodies

SKU/Catalog Number

A01674-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FGR Antibody Picoband™ catalog # A01674-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FGR Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01674-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human FGR, which shares 92% amino acid (aa) sequence identity with both mouse and rat FGR.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01674-1 is reactive to FGR in Human, Mouse, Rat

Applications

A01674-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

59 kDa

Calculated molecular weight

59.479kDa

Background of FGR

Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog, also known as FGR, is a protein which in humans is encoded by the FGR gene. This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to plasma membrane ruffles, and functions as a negative regulator of cell migration and adhesion triggered by the beta-2 integrin signal transduction pathway. Infection with Epstein-Barr virus results in the overexpression of this gene. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot,0.1-0.5μg/ml

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of FGR using anti-FGR antibody (A01674-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human SW579 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGR antigen affinity purified polyclonal antibody (Catalog # A01674-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGR at approximately 59KD. The expected band size for FGR is at 59KD.

Gene/Protein Information For FGR (Source: Uniprot.org, NCBI)

Alternative Names

c-fgr; c-src-2 proto-oncogene; c-src2; EC 2.7.10; EC 2.7.10.2; Fgr; FLJ43153; Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog; MGC75096; p55-c-fgr protein; p55c-fgr; p55-Fgr; p58c-fgr; Proto-oncogene c-Fgr; proto-oncogene tyrosine-protein kinase FGR; Src2; SRC2c-fgr protooncogene; tyrosine-protein kinase Fgr FGR SRC2, c-fgr, c-src2, p55-Fgr, p55c-fgr, p58-Fgr, p58c-fgr FGR proto-oncogene, Src family tyrosine kinase tyrosine-protein kinase Fgr|Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog|c-fgr protooncogene|c-src-2 proto-oncogene|feline Gardner-Rasheed sarcoma viral oncogene homolog|p55-c-fgr protein|proto-oncogene c-Fgr|proto-oncogene tyrosine-protein kinase FGR|v-fgr feline Gardner-Rasheed sarcoma viral oncogene homolog

For more info on FGR, check out the FGR Infographic

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In this infographic, you will see the following information for FGR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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