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Product Info Summary
| SKU: | A01675 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Fibroblast growth factor 4 FGF4 Antibody
SKU/Catalog Number
A01675
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Fibroblast growth factor 4 FGF4 Antibody (Catalog # A01675). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
FGF4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Fibroblast growth factor 4 FGF4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01675)
Host
Rabbit
Contents
FGF4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
FGF4 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of the human FGF4. The immunogen is located within the last 50 amino acids of FGF4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
SOX17 antibody is human, mouse and rat reactive. SOX17 is predicted to not cross-react with other SOX proteins.
Reactive Species
A01675 is reactive to FGF4 in Human, Mouse
Applications
A01675 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
22.048kDa
Background of FGF-4
Fibroblast growth factor 4 (FGF4) is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities and play key roles in growth and survival of stem cells during embryogenesis, tissue regeneration, and carcinogenesis. FGF4 was identified by its strong oncogenic transforming activity and is a potent angiogenic factor, expressed in several highly vascularized tumors and also in adult mouse testis, intestine, and brain. Studies on the mouse homolog suggests a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. Furthermore, FGF4 regulates neural progenitor cell proliferation and neuronal differentiation. Recent studies show a growth-promoting role for FGF4 in human embryonic stem cells and a putative feedback inhibition mechanism by a novel FGF4 splice isoform that may serve to promote differentiation at a later stages of development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
FGF4 antibody can be used for detection of FGF4 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of FGF4 in NIH 3T3 cell lysate with FGF4 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunocytochemistry of FGF4 in 3T3 cells with FGF4 antibody at 2.5 μg/mL.
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Immunofluorescence of FGF4 in 3T3 cells with FGF4 antibody at 2.5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For FGF4 (Source: Uniprot.org, NCBI)
Gene Name
FGF4
Full Name
Fibroblast growth factor 4
Weight
22.048kDa
Superfamily
heparin-binding growth factors family
Alternative Names
FGF4; FGF-4; fibroblast growth factor 4; HBGF-4; HBGF-4Transforming protein KS3; heparin secretory transforming protein 1; Heparin secretory-transforming protein 1; Heparin-binding growth factor 4; HST-1; HST-1HSTF-1; HSTF1fibroblast growth factor 4 splice isoform; HSTFGF-4; human stomach cancer, transforming factor from FGF-related oncogene; kaposi sarcoma oncogene; KFGF; K-FGF; KS3; oncogene HST FGF4 FGF-4, HBGF-4, HST, HST-1, HSTF-1, HSTF1, K-FGF, KFGF fibroblast growth factor 4 fibroblast growth factor 4|heparin secretory transforming protein 1|heparin-binding growth factor 4|human stomach cancer, transforming factor from FGF-related oncogene|kaposi sarcoma oncogene|oncogene HST|transforming protein KS3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FGF4, check out the FGF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FGF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fibroblast growth factor 4 FGF4 Antibody (A01675)
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