Click image to see more details
Product Info Summary
| SKU: | A03925 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-FLASH CASP8AP2 Antibody
SKU/Catalog Number
A03925
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-FLASH CASP8AP2 Antibody (Catalog # A03925). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
FLASH antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-FLASH CASP8AP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03925)
Host
Rabbit
Contents
FLASH Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
FLASH antibody was raised against a synthetic peptide corresponding to amino acids near the carboxy terminus of human FLASH which differ from those of mouse by one amino acid. The immunogen is located within the last 50 amino acids of FLASH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
IFIT3 antibody is human specific. This antibody is predicted to not cross-react with other members of the IFIT protein family.
Reactive Species
A03925 is reactive to CASP8AP2 in Human
Applications
A03925 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
222.658kDa
Background of FLASH
A novel mammalian CED-4 homologous was recently identified and cloned in mouse and human and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8. There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity. FLASH is widely expressed.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
FLASH antibody can be used for detection of FLASH by Western blot at 0.5 μg/mL. An approximate 220 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of FLASH in HeLa whole cell lysate with FLASH antibody at 0.5 μg/mL.
Click image to see more details
Immunocytochemistry of FLASH in HeLa cells with FLASH antibody at 10 μg/mL.
Click image to see more details
Immunofluorescence of FLASH in HeLa cells with FLASH antibody at 10 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For CASP8AP2 (Source: Uniprot.org, NCBI)
Gene Name
CASP8AP2
Full Name
CASP8-associated protein 2
Weight
222.658kDa
Alternative Names
CASP8 associated protein 2; caspase 8 associated protein 2; CED-4; FLASHKIAA1315CASP8-associated protein 2; FLICE associated huge; FLICE-associated huge protein; FLJ11208; human FLASH; RIP25FLASH homolog RIP25 CASP8AP2 CED-4, FLASH, RIP25 caspase 8 associated protein 2 CASP8-associated protein 2|FLASH homolog RIP25|FLICE associated huge|FLICE-associated huge protein|human FLASH
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP8AP2, check out the CASP8AP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP8AP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FLASH CASP8AP2 Antibody (A03925)
Hello CJ!
No publications found for A03925
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-FLASH CASP8AP2 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-FLASH CASP8AP2 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
