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Product Info Summary
| SKU: | A04517 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Flotillin 1 FLOT1 Antibody
View all Flotillin-1 Antibodies
SKU/Catalog Number
A04517
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Flotillin 1 FLOT1 Antibody catalog # A04517. Tested in WB,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Flotillin 1 FLOT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04517)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human LFNG.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04517 is reactive to FLOT1 in Human, Mouse, Rat
Applications
A04517 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
47.355kDa
Background of Flotillin-1
Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Decreases the binding of JAGGED1 to NOTCH2 but not that of DELTA1. Essential mediator of somite segmentation and patterning By similarity.
Johnston S.H., Development 124:2245-2254(1997).
Cole S.E., Dev. Cell 3:75-84(2002).
Shimizu K., J. Biol. Chem. 276:25753-25758(2001)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
IHC: 1:50-1:100
FC: 1:50-1:100
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of FLOT1 using Anti-Flotillin 1 FLOT1 Antibody (A04517).
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-Flotillin 1 FLOT1 Antibody A04517
Western blot analysis of Flotillin 1 on PC-3M cell lysate using anti-Flotillin 1 antibody at 1/500 dilution.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For FLOT1 (Source: Uniprot.org, NCBI)
Gene Name
FLOT1
Full Name
Flotillin-1
Weight
47.355kDa
Superfamily
band 7/mec-2 family
Alternative Names
flotillin 1; flotillin-1; integral membrane component of caveolae FLOT1 flotillin 1 flotillin-1|integral membrane component of caveolae
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FLOT1, check out the FLOT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FLOT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Flotillin 1 FLOT1 Antibody (A04517)
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No publications found for A04517
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Customer Reviews
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