Anti-FMO5 Antibody

Product Name

Anti-FMO5 Antibody

View all FMO5 Antibodies

SKU/Catalog Number

PA2108

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FMO5 Antibody catalog # PA2108. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FMO5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2108)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human FMO5, different from the related rat sequence by one amino acid, and different from the related mouse sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2108 is reactive to FMO5 in Human

Applications

PA2108 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

60 kDa

Calculated molecular weight

60.221kDa

Background of FMO5

Dimethylaniline monooxygenase[N-oxide-forming] 5 also known as FMO 5 is an enzyme that in humans is encoded by the FMO5 gene. By fluorescence in situ hybridization, this gene was assigned to human chromosome 1q21.1. FMO5 is not an efficient drug-metabolizing enzyme and that it may have an alternative physiologic role. FMO5 transcripts play an important role in progesterone-regulated in breast cancer cells specifically under the control of the progesterone receptor B-isoform.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of FMO5 using anti-FMO5 antibody (PA2108).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMO5 antigen affinity purified polyclonal antibody (Catalog # PA2108) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FMO5 at approximately 60 kDa. The expected band size for FMO5 is at 60 kDa.

Gene/Protein Information For FMO5 (Source: Uniprot.org, NCBI)

Alternative Names

dimethylaniline monooxygenase [N-oxide-forming] 5; Dimethylaniline oxidase 5; EC 1.14.13.8; flavin containing monooxygenase 5; FMO 5; Hepatic flavin-containing monooxygenase 5 FMO5 hBVMO1 flavin containing dimethylaniline monoxygenase 5 flavin-containing monooxygenase 5|FMO 5|NAPDH oxidase|baeyer-Villiger monooxygenase 1|dimethylaniline monooxygenase [N-oxide-forming] 5|dimethylaniline oxidase 5|flavin containing monooxygenase 5|hepatic flavin-containing monooxygenase 5

For more info on FMO5, check out the FMO5 Infographic

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In this infographic, you will see the following information for FMO5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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