Anti-FOXC1 Antibody Picoband™

Product Name

Anti-FOXC1 Antibody Picoband™

View all FOXC1 Antibodies

SKU/Catalog Number

A00962-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FOXC1 Antibody Picoband™ catalog # A00962-2. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FOXC1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00962-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human FOXC1 recombinant protein (Position: T392-F554).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00962-2 is reactive to FOXC1 in Human

Applications

A00962-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

75 kDa

Calculated molecular weight

56.789kDa

Background of FOXC1

Forkhead box C1, also known as FOXC1, is a protein which in humans is encoded by the FOXC1 gene. It is mapped to 6p25.3. This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25μg/ml, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of FOXC1 using anti-FOXC1 antibody (A00962-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXC1 antigen affinity purified polyclonal antibody (Catalog # A00962-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXC1 at approximately 75KD. The expected band size for FOXC1 is at 57KD.

Gene/Protein Information For FOXC1 (Source: Uniprot.org, NCBI)

Alternative Names

ARA; FKHL7; FKHL7RIEG3; forkhead box C1; forkhead box protein C1; forkhead, drosophila, homolog-like 7; forkhead/winged helix-like transcription factor 7; forkhead-related activator 3; Forkhead-related protein FKHL7; Forkhead-related transcription factor 3; FoxC1; FREAC3; FREAC-3; FREAC3IRID1; IGDA; IHG1; IRID1; myeloid factor-delta; RIEG3 FOXC1 ARA, ASGD3, FKHL7, FREAC-3, FREAC3, IGDA, IHG1, IRID1, RIEG3 forkhead box C1 forkhead box protein C1|forkhead box C1 protein|forkhead, drosophila, homolog-like 7|forkhead-related activator 3|forkhead-related protein FKHL7|forkhead-related transcription factor 3|forkhead/winged helix-like transcription factor 7|myeloid factor-delta

For more info on FOXC1, check out the FOXC1 Infographic

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In this infographic, you will see the following information for FOXC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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