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Product Info Summary
| SKU: | A01780-3 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-FOXC2 Antibody Picoband™
SKU/Catalog Number
A01780-3
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-FOXC2 Antibody Picoband™ catalog # A01780-3. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-FOXC2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01780-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Clone Number
1B9
Isotype
IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human FOXC2, which shares 88.9% amino acid (aa) sequence identity with mouse and rat FOXC2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01780-3 is reactive to FOXC2 in Human
Applications
A01780-3 is guaranteed for IF, ICC, WB Boster Guarantee
Observed Molecular Weight
54 kDa
Calculated molecular weight
53.719kDa
Background of FOXC2
Forkhead box protein C2 (FOXC2) also known as forkhead-related protein FKHL14 (FKHL14), transcription factor FKH-14, or mesenchyme fork head protein 1 (MFH1) is a protein that in humans is encoded by the FOXC2 gene. This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FOXC2 using anti-FOXC2 antibody (A01780-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Hela nuclear lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXC2 antigen affinity purified polyclonal antibody (Catalog # A01780-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXC2 at approximately 54 kDa. The expected band size for FOXC2 is at 54 kDa.
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Figure 2. IF analysis of FOXC2 using anti-FOXC2 antibody (A01780-3) and anti-Beta Tubulin antibody (M01857-3).
FOXC2 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FOXC2 Antibody (A01780-3) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and FITC Conjugated Goat Anti-Mouse IgG (BA1101) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For FOXC2 (Source: Uniprot.org, NCBI)
Gene Name
FOXC2
Full Name
Forkhead box protein C2
Weight
53.719kDa
Alternative Names
Fkh14; FKHL14LD; forkhead box C2 (MFH-1, mesenchyme forkhead 1); forkhead, Drosophila, homolog-like 14; Forkhead-related protein FKHL14; FoxC2; LD; Mesenchyme fork head protein 1; mesenchyme forkhead 1; MFH-1 protein; MFH1; MFH-1; MFH1forkhead box protein C2; Transcription factor FKH-14 FOXC2 FKHL14, LD, MFH-1, MFH1 forkhead box C2 forkhead box protein C2|MFH-1,mesenchyme forkhead 1|forkhead box C2 (MFH-1, mesenchyme forkhead 1)|forkhead, Drosophila, homolog-like 14|forkhead-related protein FKHL14|mesenchyme fork head protein 1|mesenchyme forkhead 1|transcription factor FKH-14
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOXC2, check out the FOXC2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOXC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FOXC2 Antibody Picoband™ (A01780-3)
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No publications found for A01780-3
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