Anti-FOXP1 Monoclonal Antibody

FOXP1 antibody

Boster Bio Anti-FOXP1 Monoclonal Antibody catalog # M00723-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00723-1
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

Product Name

Anti-FOXP1 Monoclonal Antibody

View all FOXP1 Antibodies

SKU/Catalog Number

M00723-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-FOXP1 Monoclonal Antibody catalog # M00723-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FOXP1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00723-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AFFD-6

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human FOXP1 Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B cell development.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00723-1 is reactive to FOXP1 in Human, Mouse, Rat

Applications

M00723-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

90 kDa

Calculated molecular weight

75.317kDa

Background of FOXP1

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:100-1:500
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For FOXP1 (Source: Uniprot.org, NCBI)

Gene Name

FOXP1

Full Name

Forkhead box protein P1

Weight

75.317kDa

Alternative Names

FLJ23741; fork head-related protein like B; forkhead box P1; forkhead box protein P1; FoxP1; glutamine-rich factor 1; hFKH1B; HSPC215; MGC12942; MGC88572; MGC99551; QRF1; QRF112CC4 FOXP1 12CC4, HSPC215, MFH, QRF1, hFKH1B forkhead box P1 forkhead box protein P1|fork head-related protein like B|glutamine-rich factor 1|mac-1-regulated forkhead

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on FOXP1, check out the FOXP1 Infographic

FOXP1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for FOXP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-FOXP1 Monoclonal Antibody

Question

I was wanting to use your anti-FOXP1 Monoclonal antibody for WB for mouse embryonic head tongue on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse embryonic head tongue identification?

Verified Customer

Verified customer

Asked: 2019-10-22

Answer

As indicated on the product datasheet, M00723-1 anti-FOXP1 Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse embryonic head tongue in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-22

Question

My lab would like to test anti-FOXP1 Monoclonal antibody M00723-1 on mouse embryonic head tongue for research purposes, then I may be interested in using anti-FOXP1 Monoclonal antibody M00723-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-10-14

Answer

The products we sell, including anti-FOXP1 Monoclonal antibody M00723-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-14

Question

Will M00723-1 anti-FOXP1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-08-01

Answer

You can see on the product datasheet, M00723-1 anti-FOXP1 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-08-01

Question

Do you have a BSA free version of anti-FOXP1 Monoclonal antibody M00723-1 available?

Verified Customer

Verified customer

Asked: 2019-07-31

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FOXP1 Monoclonal antibody M00723-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-31

Question

Will anti-FOXP1 Monoclonal antibody M00723-1 work for WB with embryonic head tongue?

M. Miller

Verified customer

Asked: 2019-02-07

Answer

According to the expression profile of embryonic head tongue, FOXP1 is highly expressed in embryonic head tongue. So, it is likely that anti-FOXP1 Monoclonal antibody M00723-1 will work for WB with embryonic head tongue.

Boster Scientific Support

Answered: 2019-02-07

Question

Does anti-FOXP1 Monoclonal antibody M00723-1 work on bovine IF with lung?

Verified Customer

Verified customer

Asked: 2019-02-06

Answer

Our lab technicians have not validated anti-FOXP1 Monoclonal antibody M00723-1 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-FOXP1 Monoclonal antibody M00723-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine lung in IF, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-02-06

Order DetailsPrice
M00723-1

100μL

$370
M00723-1-30ul

30μL sample

$99
M00723-1-carrier-free

Carrier Free

$370

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Free Secondary Antibody

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M00723-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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