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- Table of Contents
FRMD6 antibody (A08826-2) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of A431 cells using anti-FRMD6 antibody (A08826-2).
Overlay histogram showing A431 cells stained with A08826-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FRMD6 Antibody (A08826-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing A431 cells stained with A08826-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FRMD6 Antibody (A08826-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-FRMD6/Willin Antibody Picoband®
FRMD6 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
FRMD6 antibody was positively detected by WB in:
human Hela whole cell, human PC-3 whole cell, human PANC-1 whole cell, human CACO-2 whole cell, rat PC-12 whole cell, mouse Neuro-2a whole cell,
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
FRMD6 antibody was positively detected by IHC in:
human rectum cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
FRMD6 antibody was positively detected by ICC/IF in:
A431 cell
FRMD6 antibody was positively detected by FC in:
A431 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-FRMD6/Willin Antibody Picoband®
View all FRMD6 Antibodies
View all FRMD6 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human FRMD6/Willin recombinant protein (Position: M1-E95).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
72 kDa
Calculated Molecular Weight
72.0 kDa
Background of FRMD6
FERM domain-containing protein 6 is a protein that in humans is encoded by the FRMD6 gene. It is mapped to 14q22.1.
Cite This Product
Anti-FRMD6/Willin Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08826-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
99%
High Homology
Mouse
92%
High Homology
Rat
87%
Moderate Homology
Zebrafish
75%
Moderate Homology
Drosophila
21%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for FRMD6 antibody
Immunohistochemistry (IHC) protocol for FRMD6 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for FRMD6 antibody
Flow Cytometry (FC) protocol for FRMD6 antibody
Publications
Application:
Species:
L-Theanine Activates the mTOR Signaling Pathway through TAS1R1-FRMD6 To Promote Milk Synthesis in Bovine Mammary Epithelial Cells
Published: 2026-05-05
Journal: JOURNAL OF NUTRITIONAL BIOCHEMISTRY
Application: WB,CoIP
Impact Factor: 5.20
Species: Cow
PMID: 42097464
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Product Promise
We promise Anti-FRMD6/Willin Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A08826-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026