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Product Info Summary
| SKU: | A32410-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-FYB FYB1 Antibody
View all FYB/ADAP/SLAP130 Antibodies
SKU/Catalog Number
A32410-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-FYB FYB1 Antibody catalog # A32410-1. Tested in WB,IHC,Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FYB FYB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32410-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human GRK2 (Phospho-Tyr86)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A32410-1 is reactive to FYB1 in Human
Applications
A32410-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
85.387kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of FYB1 using Anti-FYB FYB1 Antibody (A32410-1).
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FYB antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature
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Figure 1. Western blotting validation for Anti-FYB FYB1 Antibody A32410-1
Western blot analysis of FYB on U937 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG -HRP Secondary Antibody (HA1001) at 1:5Protein Target Info & Infographic
Gene/Protein Information For FYB1 (Source: Uniprot.org, NCBI)
Gene Name
FYB1
Full Name
FYN-binding protein 1
Weight
85.387kDa
Alternative Names
ADAP; Adhesion and degranulation promoting adaptor protein; adhesion and degranulation-promoting adaptor protein; FYB; FYB-120/130SLAP-130FYN-binding protein (FYB-120/130); FYN binding protein; FYN-binding protein; FYN-T-binding protein; p120/p130; PRO0823; SLAP130; SLP-76-associated phosphoprotein FYB1 ADAP, FYB, PRO0823, SLAP-130, SLAP130, THC3 FYN binding protein 1 FYN-binding protein 1|FYB-120/130|FYN-T-binding protein|SLP-76-associated phosphoprotein|adhesion and degranulation-promoting adaptor protein|p120/p130
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FYB1, check out the FYB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FYB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FYB FYB1 Antibody (A32410-1)
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No publications found for A32410-1
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