Anti-G-protein coupled receptor 30/GPER1 Antibody Picoband®

Product Name

Anti-G-protein coupled receptor 30/GPER1 Antibody Picoband®

View all GPER1 Antibodies

SKU/Catalog Number

PA2235
BA3433-2 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-G-protein coupled receptor 30/GPER1 Antibody catalog # PA2235. Tested in WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-G-protein coupled receptor 30/GPER1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2235)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human G-protein coupled receptor 30, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2235 is reactive to GPER1 in Human, Monkey

Observed Molecular Weight

60 kDa

Calculated molecular weight

42.2 kDa

Background of GPER1

G-protein coupled estrogen receptor 1 (GPER1), also known as the GPR30 or CEPR is a G protein-coupled receptor that in humans is encode d by the GPER gene. This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. It is mapped to 7p22.3. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PA2235 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Monkey

Positive Control

WB: COLO320 Cell, MCF-7 Cell, COS7 Cell

Validation Images & Assay Conditions

See full target information GPER1

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis