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Product Info Summary
| SKU: | PB9945 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-GADD45A Antibody Picoband®
SKU/Catalog Number
PB9945
PB0989 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GADD45A Antibody Picoband® catalog # PB9945. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GADD45A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9945)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human GADD45A, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9945 is reactive to GADD45A in Human
Observed Molecular Weight
19 kDa
Calculated molecular weight
18.3 kDa
Background of GADD45A
Growth arrest and DNA-damage-inducible protein GADD45 alpha (GADD45A) is a protein that in humans is encoded by the GADD45A gene. This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. It is located on 1p34-p12. Sequence analysis of human and hamster cDNA clones demonstrated that the gene has been highly conserved and encodes a novel 165-amino acid polypeptide. Northern blot analysis detected moderate expression of a 1.4-kb GADD45A transcript in heart, skeletal muscle, and kidney, with little or no expression in brain, placenta, lung, liver, and pancreas. In addition, Gadd45a promotes epigenetic gene activation by repair-mediated DNA demethylation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9945 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: MCF-7 whole cell
Validation Images & Assay Conditions
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Western blot analysis of GADD45A using anti-GADD45A antibody (PB9945).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD45A antigen affinity purified polyclonal antibody (Catalog # PB9945) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD45A at approximately 19 kDa. The expected band size for GADD45A is at 18 kDa.
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The mechanism behind the LDEVs effect on gastric cancer cells. a KEGG pathway analysis of SGC-7901 cells after LDEVs treatment for 6 h and b 12 h. The highest KEGG pathways were labeled by red line respectively. c RT-PCR assay the relative expression of GADD45A in AGS, BGC-823, and SGC-7901 cells treated with LDEVs; d Western blot analysis of GADD45α protein expression in three gastric cancer cells; e Flow cytometry assay the intracellular ROS levels using DCFH-DA; f Cell viability of control group, LDEVs group, NAC + LDEVs group, and NAC groups respectively (* p < 0.05; ** p < 0.01, *** p < 0.001, **** p < 0.0001)
Index in PubMed under a CC BY license. PMID: 32690102
Specific Publications For Anti-GADD45A Antibody Picoband® (PB9945)
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Customer Q&As
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3 Customer Q&As for Anti-GADD45A Antibody Picoband®
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-GADD45A antibody PB9945. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-05-28
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-28
Question
Will anti-GADD45A antibody PB9945 work for WB with lung?
Verified Customer
Verified customer
Asked: 2018-01-24
Answer
According to the expression profile of lung, GADD45A is highly expressed in lung. So, it is likely that anti-GADD45A antibody PB9945 will work for WB with lung.
Boster Scientific Support
Answered: 2018-01-24
Question
We are currently using anti-GADD45A antibody PB9945 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
J. Miller
Verified customer
Asked: 2016-03-08
Answer
The anti-GADD45A antibody (PB9945) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-03-08
