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Product Info Summary
| SKU: | M01634 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-GALC Antibody (N-Term)
View all Galactosylceramidase/GALC Antibodies
SKU/Catalog Number
M01634
Size
50 µl
Description
Boster Bio Anti-GALC Antibody (N-Term) (Catalog # M01634). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-GALC Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01634)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This GALC antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 26-56 amino acids from human GALC.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01634 is reactive to GALC in Human
Applications
M01634 is guaranteed for Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
77.063kDa
Background of Galactosylceramidase/GALC
Hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Enzyme with very low activity responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney and epithelial cells of small intestine and colon.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-GALC Antibody (N-Term) at 1:2000 dilution
Lane 1: human brain lysate
Lane 2: Hela whole cell lysate
Lane 3: NCI-H1299 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 77 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing Hela cells stained with M01634 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M01634, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For GALC (Source: Uniprot.org, NCBI)
Gene Name
GALC
Full Name
Galactocerebrosidase
Weight
77.063kDa
Superfamily
glycosyl hydrolase 59 family
Alternative Names
EC 3.2.1.46; Galactocerebrosidase; Galactocerebroside beta-galactosidase; galactosylceramidase (Krabbe disease); Galactosylceramidase; Galactosylceramide beta-galactosidase; Galactosylceraminidase; GALC; GALCERase GALC galactosylceramidase galactocerebrosidase|GALCERase|galactocerebroside beta-galactosidase|galactosylceramide beta-galactosidase|galactosylceraminidase|testis tissue sperm-binding protein Li 88E|testis tissue sperm-binding protein Li 89A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GALC, check out the GALC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GALC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GALC Antibody (N-Term) (M01634)
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No publications found for M01634
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