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Product Info Summary
| SKU: | A05109-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-GAT1/SLC6A1 Antibody Picoband®
SKU/Catalog Number
A05109-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-GAT1/SLC6A1 Antibody Picoband® catalog # A05109-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-GAT1/SLC6A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05109-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human GAT1/SLC6A1 recombinant protein (Position: A23-A215). Human SLC6A1 shares 98.4% amino acid (aa) sequence identity with both mouse and rat SLC6A1.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05109-2 is reactive to SLC6A1 in Human, Mouse, Rat
Observed Molecular Weight
67 kDa
Calculated molecular weight
67.1 kDa
Background of SLC6A1
GABA transporter 1 (GAT1), also known as sodium- and chloride-dependent GABA transporter 1, is a protein that in humans is encoded by the SLC6A1 gene. GABA Transporter 1 uses Na+ and Cl- to create a gradient, which removes or adds GABA to extracellular spaces in the cerebrum and cerebellum. The stoichiometry for GABA Transporter 1 is 2 Na+: 1 Cl-: 1 GABA. The activity of GAT1 is largely dependent on the presence of Na+, while Cl- assists by increasing the ability for GAT-1 to uptake GABA.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05109-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, rat C6 whole cell, mouse Neuro-2a whole cell
Validation Images & Assay Conditions
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Western blot analysis of GAT1/SLC6A1 using anti-GAT1/SLC6A1 antibody (A05109-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: rat C6 whole cell lysates,
Lane 3: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAT1/SLC6A1 antigen affinity purified polyclonal antibody (Catalog # A05109-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAT1/SLC6A1 at approximately 67 kDa. The expected band size for GAT1/SLC6A1 is at 67 kDa.
Specific Publications For Anti-GAT1/SLC6A1 Antibody Picoband® (A05109-2)
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