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Product Info Summary
| SKU: | M01415 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GATA2/3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01415
BM4342 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GATA2/3 Rabbit Monoclonal Antibody catalog # M01415. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GATA2/3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01415)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DHA-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GATA2/3
Reactive Species
M01415 is reactive to GATA2 in Human, Mouse, Rat
Observed Molecular Weight
51 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01415 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: human K562 whole cell, human Hela whole cell
IHC: mouse kidney tissue
ICC/IF: K562 cell
Validation Images & Assay Conditions
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Western blot analysis of GATA2/3 using anti-GATA2/3 antibody (M01415).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA2/3 antigen affinity purified monoclonal antibody (M01415) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GATA2/3 at approximately 51 kDa. The expected band size for GATA2/3 is at 51 kDa.
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Immunohistochemical analysis of paraffin-embedded mouse kidney, using GATA2/3 Antibody.
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Immunofluorescent analysis of K562 cells, using GATA2/3 Antibody .
Specific Publications For Anti-GATA2/3 Rabbit Monoclonal Antibody (M01415)
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Customer Q&As
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16 Customer Q&As for Anti-GATA2/3 Rabbit Monoclonal Antibody
Question
We ordered your anti-GATA2/3 Rabbit Monoclonal antibody for WB on colon carcinoma last year. I am using human, and We intend to use the antibody for IF next. We need examining colon carcinoma as well as endometrium in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
I viewed the website and datasheets of our anti-GATA2/3 Rabbit Monoclonal antibody and I see that M01415 has been validated on human in both WB and IF. Thus M01415 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-30
Question
I see that the anti-GATA2/3 Rabbit Monoclonal antibody M01415 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-02-24
Question
Does anti-GATA2/3 Rabbit Monoclonal antibody M01415 work for IP with brain placenta?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
According to the expression profile of brain placenta, GATA2 is highly expressed in brain placenta. So, it is likely that anti-GATA2/3 Rabbit Monoclonal antibody M01415 will work for IP with brain placenta.
Boster Scientific Support
Answered: 2020-02-11
Question
Would M01415 anti-GATA2/3 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
You can see on the product datasheet, M01415 anti-GATA2/3 Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-05
Question
My lab would like to test anti-GATA2/3 Rabbit Monoclonal antibody M01415 on human brain placenta for research purposes, then I may be interested in using anti-GATA2/3 Rabbit Monoclonal antibody M01415 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
The products we sell, including anti-GATA2/3 Rabbit Monoclonal antibody M01415, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-29
Question
We need using your anti-GATA2/3 Rabbit Monoclonal antibody for pituitary gland development studies. Has this antibody been tested with western blotting on k562 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
We appreciate your inquiry. This M01415 anti-GATA2/3 Rabbit Monoclonal antibody is tested on mouse kidney, k562 cell lysate. It is guaranteed to work for IP, IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-12-13
Question
I was wanting to use your anti-GATA2/3 Rabbit Monoclonal antibody for IP for human brain placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain placenta identification?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
It shows on the product datasheet, M01415 anti-GATA2/3 Rabbit Monoclonal antibody has been tested for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-15
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain placenta using anti-GATA2/3 Rabbit Monoclonal antibody M01415. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-15
Question
Is this M01415 anti-GATA2/3 Rabbit Monoclonal antibody reactive to the isotypes of GATA2?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
The immunogen of M01415 anti-GATA2/3 Rabbit Monoclonal antibody is A synthesized peptide derived from human GATA2/3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-29
Question
Is a blocking peptide available for product anti-GATA2/3 Rabbit Monoclonal antibody (M01415)?
K. Li
Verified customer
Asked: 2019-02-18
Answer
We do provide the blocking peptide for product anti-GATA2/3 Rabbit Monoclonal antibody (M01415). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-02-18
Question
Can you help my question with product M01415, anti-GATA2/3 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-12-20
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01415 anti-GATA2/3 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-20
Question
We were well pleased with the WB result of your anti-GATA2/3 Rabbit Monoclonal antibody. However we have been able to see positive staining in endothelial cell nucleus. using this antibody. Is that expected? Could you tell me where is GATA2 supposed to be expressed?
P. Brown
Verified customer
Asked: 2018-11-27
Answer
From literature, endothelial cell does express GATA2. Generally GATA2 expresses in nucleus. Regarding which tissues have GATA2 expression, here are a few articles citing expression in various tissues:
Brain, and Placenta, Pubmed ID: 15489334
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Endothelial cell, Pubmed ID: 1370462
Placenta, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-11-27
Question
See attached the WB image, lot number and protocol we used for brain placenta using anti-GATA2/3 Rabbit Monoclonal antibody M01415. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-20
Question
We are currently using anti-GATA2/3 Rabbit Monoclonal antibody M01415 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
T. Wu
Verified customer
Asked: 2018-02-13
Answer
The anti-GATA2/3 Rabbit Monoclonal antibody (M01415) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-13
Question
We have been able to see staining in mouse placenta. What should we do? Is anti-GATA2/3 Rabbit Monoclonal antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2017-11-14
Answer
From literature placenta does express GATA2. From Uniprot.org, GATA2 is expressed in endometrium, endothelial cell, placenta, brain placenta, cervix carcinoma erythroleukemia, colon carcinoma, among other tissues. Regarding which tissues have GATA2 expression, here are a few articles citing expression in various tissues:
Brain, and Placenta, Pubmed ID: 15489334
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Endothelial cell, Pubmed ID: 1370462
Placenta, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-11-14
Question
Is there a BSA free version of anti-GATA2/3 Rabbit Monoclonal antibody M01415 available?
S. Jackson
Verified customer
Asked: 2015-02-26
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-GATA2/3 Rabbit Monoclonal antibody M01415 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-02-26
