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Product Info Summary
| SKU: | A07110-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GBP5 Antibody
SKU/Catalog Number
A07110-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-GBP5 Antibody catalog # A07110-1. Tested in WB, IHC, ICC, IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-GBP5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07110-1)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human GBP5 recombinant protein (Position: S377-L586).
Reactive Species
A07110-1 is reactive to GBP5 in Human, Mouse
Observed Molecular Weight
67 kDa
Calculated molecular weight
66.6 kDa
Background of GBP5
This gene belongs to the TRAFAC class dynamin-like GTPase superfamily. The encoded protein acts as an activator of NLRP3 inflammasome assembly and has a role in innate immunity and inflammation. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07110-1 is guaranteed for ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-400
Immunocytochemistry/Immunofluorescence, 1:50-400
Immunoprecipitation, 1:50
Flow Cytometry (Fixed), 1-3μg/1x106 cells
ELISA, 1:100-1000
Positive Control
WB: human Jurkat whole cell, human U937 whole cell, human U251 whole cell, mouse spleen tissue, mouse RAW2647 whole cell
IHC: human breast cancer tissue, human testis cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of GBP5 using anti-GBP5 antibody (A07110-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human U937 whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: mouse spleen tissue lysates,
Lane 5: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GBP5 antigen affinity purified polyclonal antibody (A07110-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GBP5 at approximately 67 kDa. The expected band size for GBP5 is at 67 kDa.
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