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- Table of Contents
GCLM antibody (M02948-2) performs well in IP, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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IHC analysis of GCLM using anti-GCLM antibody (M02948-2).
GCLM was detected in a paraffin-embedded section of human clear cell renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GCLM Antibody (M02948-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
GCLM was detected in a paraffin-embedded section of human clear cell renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GCLM Antibody (M02948-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-GCLM Rabbit Monoclonal Antibody
GCLM antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IP, IHC, WB |
Tested Applications
GCLM antibody was positively detected by WB in:
human Hela whole cell, human A549 whole cell, human Jurkat whole cell, human K562 whole cell, rat liver tissue, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GCLM antibody was positively detected by IHC in:
human clear cell renal cell carcinoma tissue, human clear cell renal cell carcinoma tissue, human liver cancer tissue, human liver cancer tissue, human ovarian cancer tissue, human ovarian cancer tissue, human placenta tissue, human placenta tissue, human rectum adenocarcinoma tissue, human rectum adenocarcinoma tissue, human thyroid papillary carcinoma tissue, human thyroid papillary carcinoma tissue, human urothelial carcinoma tissue, human urothelial carcinoma tissue, mouse adrenal tissue, mouse adrenal tissue, mouse kidney tissue, mouse kidney tissue, rat kidney tissue, rat kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
IP 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Product Information
Product Name
Anti-GCLM Rabbit Monoclonal Antibody
View all GCLM Antibodies
View all GCLM Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
28G35
Isotype
IgG
Immunogen
A synthesized peptide derived from human GCLM
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
28 kDa
Calculated Molecular Weight
30.7 kDa
Cite This Product
Anti-GCLM Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02948-2)
Protocols
Western Blot protocol for GCLM antibody
Immunohistochemistry (IHC) protocol for GCLM antibody
Publications
Application:
Species:
SPOCK1 acts as a negative regulator of ferroptosis in non-small cell lung cancer
Published: 2026-05-14
Journal: npj Precision Oncology
Application: WB
Impact Factor: 9.90
Species: Human
PMID: 42135411
IER3 Promotes Non-Small Cell Lung Cancer Malignancy by Suppressing Ferroptosis via the AKT/GSK3β/NRF2 Pathway
Published: 2026-01-23
Journal: FREE RADICAL BIOLOGY AND MEDICINE
Application: WB
Impact Factor: 8.20
Species: Human
PMID: 41581578
Review
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Product Promise
We promise Anti-GCLM Rabbit Monoclonal Antibody performs excellently in relevant applications for IP, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M02948-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026