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- Table of Contents
eIF-2-alpha kinase GCN2 Antibodies
AND Eif2ak4 ELISA kits, eIF-2-alpha kinase GCN2 Proteins
Many biological assays use antibodies to detect eIF-2-alpha kinase GCN2, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with eIF-2-alpha kinase GCN2 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop eIF-2-alpha kinase GCN2 antibodies...
Designing a Western blot for eIF-2-alpha kinase GCN2? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the EIF2AK4 Western Blot GuideEif2ak4 Infographic by Boster Bio
All Eif2ak4 Products
Product
Figures
Specificity
Applications
Price
Human, Monkey, Mouse
ELISA, Flow Cytometry, IHC, WB
$370
Cat # A01172-2
100 μg/vial
Cat # A01172-2
100 μg/vial
Anti-GCN2 EIF2AK4 Rabbit Monoclonal Antibody
1 Citations
Human
Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # M01172
100 μl
Cat # M01172
100 μl
Human, Mouse, Rat
IF, IHC, ICC, WB
$405
Cat # A01172
100ul
Cat # A01172
100ul
Eif2ak4 Overview
Facts about eIF-2-alpha kinase GCN2.
eIF-2-alpha kinase GCN2 (Eif2ak4)
Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on'Ser-52' in response to low amino acid availability (PubMed:10504407, PubMed:10655230, PubMed:12176355, PubMed:12215525, PubMed:15213227, PubMed:16054071, PubMed:16176978, PubMed:16121183, PubMed:15774759, PubMed:16601681, PubMed:26102367). Plays a role as an activator of the integrated stress response (ISR) required for adapatation to amino acid starvation.
Converts phosphorylated eIF-2-alpha/EIF2S1 either to a competitive inhibitor of the translation initiation factor eIF-2B, leading to a global protein synthesis repression, and thus to a reduced overall use of amino acids, or into a translational initiation activation of specific mRNAs, such as the transcriptional activator ATF4, and hence enabling ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion (PubMed:10655230, PubMed:11106749, PubMed:12176355, PubMed:15213227, PubMed:16176978, PubMed:26102367). Binds uncharged tRNAs (By similarity).
Gene Information
| Mouse | |
|---|---|
| Gene Name: | Eif2ak4 |
| Uniprot: | Q9QZ05 |
| Entrez: | 27103 |
Family
| Belongs to: |
|---|
| protein kinase superfamily |
Alternative Names
EIF2AK4; eukaryotic translation initiation factor 2 alpha kinase 4; eukaryotic translation initiation factor 2-alpha kinase 4; GCN2; GCN2GCN2 eIF2alpha kinase; GCN2-like protein; KIAA1338EC 2.7.11.1
Molecular Weight
Mass (kDA):
186.487 kDA
Genomic Context
| Mouse | |
|---|---|
| Location: | 2|2 E5 |
| Sequence: | 2; |
Tissue Specificity
Expressed in liver (PubMed:10504407). Expressed predominantly in the hippocampal CA1 region and the dentate gyrus, and to a lesser degree in CA3 (at protein level) (PubMed:16121183). Expressed in liver, lung, brain, kidney, skeletal muscle and testis (PubMed:10504407, PubMed:10655230). Expressed weakly in heart and spleen (PubMed:10655230). Expressed in the hippocampal CA1 and CA3 regions, the dentate gyrus and cerebellum (PubMed:16121183). Isoform 1 is widely expressed (PubMed:12215525). Isoform 1 is expressed in brain, liver, skeletal muscle and testis (PubMed:10655230). Isoform 3 is expressed in lung, brain, testis, prostate and choroid plexus (PubMed:12215525). Isoform 4 is expressed in muscle, lung, kidney, brain, testis and prostate (PubMed:10655230, PubMed:12215525).
Diseases
- Neoplasms
- Malignant Neoplasms
- Decreased Immunologic Activity [pe]
- Autoimmunity
- Malnutrition
- Physiological Stress
- Virus Diseases
- Endoplasmic Reticulum Stress
- Liver Carcinoma
- Tumor Progression
- Hypoxia
- Malignant Paraganglionic Neoplasm
Pathways
- Translation
- Cell Cycle
- Autophagy
- Transport
- Cell Death
- Secretion
- Immune Response
- Regulation Of Gene Expression
- Response To Uv
- Feeding Behavior
- Long-term Memory
- Response To Stress
- Cell Proliferation
PTMs
- Phosphorylation
- Cleavage
Research Areas
- Phospho-Specific
- Protein Kinase
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Eif2ak4
bosterbio.com/bosterbio-gene-info-cards/Eif2ak4
References
PMID: 10504407 by Berlanga J.J., et al. Characterization of a mammalian homolog of the GCN2 eukaryotic initiation factor 2alpha kinase.
PMID: 10655230 by Sood R., et al. A mammalian homologue of GCN2 protein kinase important for translational control by phosphorylation of eukaryotic initiation factor-2alpha.