Anti-GIGYF2 Antibody Picoband™

Product Name

Anti-GIGYF2 Antibody Picoband™

View all PERQ2 Antibodies

SKU/Catalog Number

A04303-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GIGYF2 Antibody Picoband™ catalog # A04303-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-GIGYF2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04303-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human GIGYF2 recombinant protein (Position: R413-Y1299).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04303-2 is reactive to GIGYF2 in Human

Applications

A04303-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

180 kDa

Calculated molecular weight

150.07kDa

Background of PERQ2

This gene contains CAG trinucleotide repeats and encodes a protein containing several stretches of polyglutamine residues. The encoded protein may be involved in the regulation of tyrosine kinase receptor signaling. This gene is located in a chromosomal region that was genetically linked to Parkinson disease type 11, and mutations in this gene were thought to be causative for this disease. However, more recent studies in different populations have been unable to replicate this association. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of GIGYF2 using anti-GIGYF2 antibody (A04303-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GIGYF2 antigen affinity purified polyclonal antibody (Catalog # A04303-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GIGYF2 at approximately 180 kDa. The expected band size for GIGYF2 is at 180 kDa.

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Figure 2. IHC analysis of GIGYF2 using anti-GIGYF2 antibody (A04303-2).
GIGYF2 was detected in a paraffin-embedded section of human colorectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GIGYF2 Antibody (A04303-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 3. IHC analysis of GIGYF2 using anti-GIGYF2 antibody (A04303-2).
GIGYF2 was detected in a paraffin-embedded section of human gall bladder adenosquamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GIGYF2 Antibody (A04303-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 4. IF analysis of GIGYF2 using anti-GIGYF2 antibody (A04303-2).
GIGYF2 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GIGYF2 Antibody (A04303-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 5. Flow Cytometry analysis of JK cells using anti-GIGYF2 antibody (A04303-2).
Overlay histogram showing JK cells stained with A04303-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GIGYF2 Antibody (A04303-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For GIGYF2 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp686J17223; FLJ23368; GRB10 interacting GYF protein 2; GRB10-interacting GYF protein 2; GYF domain containing 2; GYF2; KIAA0642DKFZp686I15154; PARK11; Parkinson disease (autosomal recessive, early onset) 11; PERQ amino acid rich, with GYF domain 2; PERQ amino acid rich, with GYF domain 3; PERQ amino acid-rich with GYF domain-containing protein 2; PERQ2; PERQ3; TNRC15; trinucleotide repeat containing 15; Trinucleotide repeat-containing gene 15 protein GIGYF2 GYF2, PARK11, PERQ2, PERQ3, TNRC15 GRB10 interacting GYF protein 2 GRB10-interacting GYF protein 2|PERQ amino acid rich, with GYF domain 3|PERQ amino acid-rich with GYF domain-containing protein 2|Parkinson disease (autosomal recessive, early onset) 11|trinucleotide repeat-containing gene 15 protein

For more info on GIGYF2, check out the GIGYF2 Infographic

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In this infographic, you will see the following information for GIGYF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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