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Product Info Summary
| SKU: | A06589 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-GluR8 GRM8 Antibody
SKU/Catalog Number
A06589
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-GluR8 GRM8 Antibody (Catalog # A06589). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GluR8 GRM8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06589)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human GluR8.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A06589 is reactive to GRM8 in Human, Mouse, Rat
Applications
A06589 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
101.741kDa
Background of mGluR8
G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity.
Patrick. M. Downey, J Biomol Screen, Dec 2005; 10: 841 - 848.
RJ Wenthold, J. Biol. Chem., Jan 1994; 269: 1332 - 1339.
Toshiaki Watanabe, Cancer Res., Oct 2006; 66: 9804 - 9808.
V. Bruno, J. Neurosci., Sep 2000; 20: 6413.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from k562 cells (Lane 2), using mGluR8 antiobdy A06589. The lane on the left is treated with synthesized peptide.
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Western blot analysis of extracts from mouse brain, using GluR8 antibody A06589.
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Immunohistochemical analysis of paraffin-embedded human brain tissue using GluR8 antibody A06589.
Protein Target Info & Infographic
Gene/Protein Information For GRM8 (Source: Uniprot.org, NCBI)
Gene Name
GRM8
Full Name
Metabotropic glutamate receptor 8
Weight
101.741kDa
Superfamily
G-protein coupled receptor 3 family
Alternative Names
FLJ41058; glutamate metabotropic receptor 8; glutamate receptor, metabotropic 8; GPRC1H; GPRC1HMGLUR8GLUR8; GRM8; metabotropic glutamate receptor 8; MGC126724; mGlu8; mGluR8 GRM8 GLUR8, GPRC1H, MGLUR8, mGlu8 glutamate metabotropic receptor 8 metabotropic glutamate receptor 8|glutamate receptor, metabotropic 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GRM8, check out the GRM8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GRM8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GluR8 GRM8 Antibody (A06589)
Hello CJ!
No publications found for A06589
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-GluR8 GRM8 Antibody
Question
We are currently using anti-GluR8 antibody A06589 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
The anti-GluR8 antibody (A06589) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-08
Question
Is there a BSA free version of anti-GluR8 antibody A06589 available?
Verified Customer
Verified customer
Asked: 2019-03-18
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-GluR8 antibody A06589 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-03-18
Question
My question regarding product A06589, anti-GluR8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Williams
Verified customer
Asked: 2018-11-14
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06589 anti-GluR8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-11-14
Question
I have attached the WB image, lot number and protocol we used for brain using anti-GluR8 antibody A06589. Please let me know if you require anything else.
J. Wu
Verified customer
Asked: 2018-11-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-08
Question
I was wanting to use your anti-GluR8 antibody for IHC for rat brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain identification?
Verified Customer
Verified customer
Asked: 2018-05-14
Answer
As indicated on the product datasheet, A06589 anti-GluR8 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-14
Question
Is this A06589 anti-GluR8 antibody reactive to the isotypes of GRM8?
P. Johnson
Verified customer
Asked: 2016-02-23
Answer
The immunogen of A06589 anti-GluR8 antibody is Synthesized peptide derived from human GluR8. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-02-23
Question
Is a blocking peptide available for product anti-GluR8 antibody (A06589)?
T. Singh
Verified customer
Asked: 2016-01-27
Answer
We do provide the blocking peptide for product anti-GluR8 antibody (A06589). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-01-27
