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Product Info Summary
| SKU: | M03191 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-Glutamine Synthetase GLUL Rabbit Monoclonal Antibody
SKU/Catalog Number
M03191
BM4196 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Glutamine Synthetase GLUL Rabbit Monoclonal Antibody catalog # M03191. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glutamine Synthetase GLUL Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03191)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CCH-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Glutamine Synthetase
Reactive Species
M03191 is reactive to GLUL in Human, Mouse, Rat
Observed Molecular Weight
42 kDa
Calculated molecular weight
42.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03191 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
FC 1:50
Positive Control
IHC: Human glioblastoma tissue, Human prostate cancer tissue, Rat kidney tissue, Mouse ovary tissue, Rat cerebral cortex tissue, human liver tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Western blot analysis of Glutamine Synthetase expression in (1)Jurkat cell lysate;(2) HeLa cell lysate.
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Immunohistochemical analysis of paraffin-embedded Human glioblastoma, using the Antibody at 1:250 dilution.
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Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:250 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat kidney, using the Antibody at 1:400 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse ovary, using the Antibody at 1:400 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex, using the Antibody at 1:400 dilution.
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Immunohistochemical analysis of paraffin-embedded human liver, using Glutamine Synthetase Antibody .
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Specific Publications For Anti-Glutamine Synthetase GLUL Rabbit Monoclonal Antibody (M03191)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Glutamine Synthetase GLUL Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-Glutamine Synthetase Rabbit Monoclonal antibody for IHC for human muscle testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human muscle testis identification?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
As indicated on the product datasheet, M03191 anti-Glutamine Synthetase Rabbit Monoclonal antibody has been tested for IP, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human muscle testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-31
Question
I see that the anti-Glutamine Synthetase Rabbit Monoclonal antibody M03191 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-03-29
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-03-29
Question
We are currently using anti-Glutamine Synthetase Rabbit Monoclonal antibody M03191 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
R. Roberts
Verified customer
Asked: 2016-07-28
Answer
The anti-Glutamine Synthetase Rabbit Monoclonal antibody (M03191) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-07-28
