Anti-Glycophorin A / CD235a (Erythrocyte Marker) Monoclonal Antibody

Product Name

Anti-Glycophorin A / CD235a (Erythrocyte Marker) Monoclonal Antibody

View all Glycophorin A Antibodies

SKU/Catalog Number

M02184

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Glycophorin A / CD235a (Erythrocyte Marker) Monoclonal Antibody (Catalog # M02184). Tested in WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Glycophorin A / CD235a (Erythrocyte Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02184)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: GYPA/280

Isotype

IgG1, kappa

Immunogen

Recombinant human full-length GYPA protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M02184 is reactive to GYPA in Human

Applications

M02184 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

16.331kDa

Background of Glycophorin A

Recognizes a sialoglycoprotein of 39kDa, identified as glycophorin A (GPA). It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus. Glycophorins A (GPA) and B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Placenta stained with Anti-Glycophorin A Mouse Monoclonal Antibody (GYPA/280).

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Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Anti-Glycophorin A Mouse Monoclonal Antibody (GYPA/280).

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Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Anti-Glycophorin A Mouse Monoclonal Antibody (GYPA/280).

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SDS-PAGE analysis purified Anti-Glycophorin A Mouse Monoclonal Antibody (GYPA/280). Confirmation of integrity and purity of antibody.

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Western Blot analysis of K562 cell lysate using Anti-Glycophorin A Mouse Monoclonal Antibody (GYPA/280).

Gene/Protein Information For GYPA (Source: Uniprot.org, NCBI)

Alternative Names

CD235a antigen; CD235a; glycophorin A (includes MN blood group); glycophorin A (MNS blood group); Glycophorin A; glycophorin Erik; glycophorin MiI; glycophorin MiIII; glycophorin MiV; glycophorin MiX; glycophorin SAT; glycophorin Sta type C; glycophorin-A; GPA; GPErik; GpMiIII; GPSAT; GYPA; HGpMiIII; HGpMiV; HGpMiX; HGpMiXI; HGpSta(C); Mi.V glycoprotein (24 AA); MN sialoglycoprotein; MNS; PAS-2; recombinant glycophorin A-B Miltenberger-DR GYPA CD235a, GPA, GPErik, GPSAT, HGpMiV, HGpMiXI, HGpSta(C), MN, MNS, PAS-2 glycophorin A (MNS blood group) glycophorin-A|MN sialoglycoprotein|Mi.V glycoprotein (24 AA)|erythroid-lineage-specific membrane sialoglycoprotein|glycophorin A (MN blood group)|glycophorin A, GPA|glycophorin Erik|glycophorin MiI|glycophorin MiV|glycophorin SAT|glycophorin Sta type C|recombinant glycophorin A-B Miltenberger-DR|sialoglycoprotein alpha

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In this infographic, you will see the following information for GYPA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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