Product Info Summary
SKU: | PA1456 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-GM-CSF/CSF2 Antibody
SKU/Catalog Number
PA1456
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GM-CSF/CSF2 Antibody catalog # PA1456. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GM-CSF/CSF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1456)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human GM-CSF.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1456 is reactive to CSF2 in Human
Applications
PA1456 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
35 kDa
Calculated molecular weight
16.295kDa
Background of GM-CSF
GM-CSF, Granulocyte-macrophage colony-stimulating factor, is a protein secreted by macrophages, T cells, mast cells, endothelial cells, and fibroblasts. By fluorescence in situ hybridization, the GM-CSF gene is mapped to 5q31.1. GM-CSF is a cytokine that functions as a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. GM-CSF is an essential regulator of neutrophil function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-GM-CSF antibody, PA1456, Western blotting
All lanes: Anti GM-CSF (PA1456) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: HT1080Whole Cell Lysate at 40ug
Predicted bind size: 16KD
Observed bind size: 35KD
Protein Target Info & Infographic
Gene/Protein Information For CSF2 (Source: Uniprot.org, NCBI)
Gene Name
CSF2
Full Name
Granulocyte-macrophage colony-stimulating factor
Weight
16.295kDa
Superfamily
GM-CSF family
Alternative Names
colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; CSF-2; GMCSF; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; Molgramostim; molgramostin; Sargramostim CSF2 CSF, GMCSF colony stimulating factor 2 granulocyte-macrophage colony-stimulating factor|colony stimulating factor 2 (granulocyte-macrophage)|granulocyte macrophage-colony stimulating factor|molgramostim|molgramostin|sargramostim
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CSF2, check out the CSF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CSF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GM-CSF/CSF2 Antibody (PA1456)
Hello CJ!
PA1456 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The Balance between Conventional DCs and Plasmacytoid DCs Is Pivotal for Immunological Tolerance during Pregnancy in the Mouse
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-GM-CSF/CSF2 Antibody
Question
We are currently using anti-GM-CSF/CSF2 antibody PA1456 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
The anti-GM-CSF/CSF2 antibody (PA1456) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-24
Question
My team were content with the WB result of your anti-GM-CSF/CSF2 antibody. However we have seen positive staining in upper lobe of left lung secreted using this antibody. Is that expected? Could you tell me where is CSF2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
From literature, upper lobe of left lung does express CSF2. Generally CSF2 expresses in secreted. Regarding which tissues have CSF2 expression, here are a few articles citing expression in various tissues:
Peripheral blood, Pubmed ID: 1737041
Boster Scientific Support
Answered: 2020-01-03
Question
We have seen staining in human peripheral blood. Any tips? Is anti-GM-CSF/CSF2 antibody supposed to stain peripheral blood positively?
Verified Customer
Verified customer
Asked: 2018-04-17
Answer
According to literature peripheral blood does express CSF2. According to Uniprot.org, CSF2 is expressed in upper lobe of left lung, peripheral blood, among other tissues. Regarding which tissues have CSF2 expression, here are a few articles citing expression in various tissues:
Peripheral blood, Pubmed ID: 1737041
Boster Scientific Support
Answered: 2018-04-17
Question
I am interested in using your anti-GM-CSF/CSF2 antibody for positive regulation of podosome assembly studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
N. Jones
Verified customer
Asked: 2016-04-27
Answer
Thank you for your inquiry. This PA1456 anti-GM-CSF/CSF2 antibody is validated on jurkat whole cell lysate, hela whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-04-27