Human GM-CSF PicoKine® Quick ELISA Kit

GM-CSF ELISA kit, 90-minute one-step protocol

Human GM-CSF PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CSF2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: FEK0364
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Assay Time: 90 Minutes

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Human GM-CSF PicoKine® Quick ELISA Kit

See all GM-CSF products

SKU/Catalog Number

FEK0364

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human GM-CSF PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CSF2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human GM-CSF PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0364)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A18-E144

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0364 is reactive to CSF2 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

FEK0364 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of GM-CSF

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.

Kit Components

Catalog numberDescription QuantityVolume
FEK0364-CAPAnti-Human GM-CSF Pre-coated 96-well Strip Microplate 112 strips of 8 wells
FEK0364-STHuman GM-CSF Standard 2 10 ng/tube
FEK0364-HHRP Linked Anti-Human CSF2 Antibody 1 6ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0220.2330.4390.7991.4152.2322.5362.516

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2821338827219407
Standard deviation1.3415.7619.781.820.1426.04
CV (%)4.8%7.4%6.4%6.7%9.2%6.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of GM-CSF in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 128303132301.474.9%
Sample 22132092102212134.712.2%
Sample 338837438843339522.255.6%
*number of samples for each test n=16.

Gene/Protein Information For CSF2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P04141

Gene ID

1437

Gene Name

CSF2

Full Name

Granulocyte-macrophage colony-stimulating factor

Weight

16.295kDa

Superfamily

GM-CSF family

Alternative Names

colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; CSF-2; GMCSF; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; Molgramostim; molgramostin; Sargramostim CSF2 CSF, GMCSF colony stimulating factor 2 granulocyte-macrophage colony-stimulating factor|colony stimulating factor 2 (granulocyte-macrophage)|granulocyte macrophage-colony stimulating factor|molgramostim|molgramostin|sargramostim

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CSF2, check out the CSF2 Infographic

CSF2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CSF2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

FEK0364 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Expression of the hGM-CSF in the silk glands of germline of gene-targeted silkworm

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3 Customer Q&As for Human GM-CSF PicoKine® Quick ELISA Kit

Question

Could you recommend any starting dilutions for this Human GM-CSF PicoKine ELISA kit? We will use BAL fluid and cell supernatant.

Verified Customer

Verified customer

Asked: 2020-02-17

Answer

We only have recommended dilution factors for serum and plasma samples since these types of samples are pretty consistent while cell culture supernatant samples varies depending on the cell type, media, treatments, culturing conditions, etc. Thus, if we give you a recommended dilution range for cell culture supernatants, all of the factors would have to be considered and the dilution range. As for BAL samples, we have not tested on it so we don

Boster Scientific Support

Answered: 2020-02-17

Question

Is FEK0364 compatible with urine samples?

Verified customer

Asked: 2020-01-20

Answer

The Human GM-CSF PicoKine™ Fast ELISA Kit (FEK0364) is used with cell culture supernates, serum and plasma(heparin, EDTA, citrate) sample types.

Boster Scientific Support

Answered: 2020-01-20

Question

To Support: Good Day, I would like to know the normal assay range of the product Human GM-CSF PicoKine ELISA kit.

Verified Customer

Verified customer

Asked: 2016-11-09

Answer

The assay range of Human GM-CSF PicoKine ELISA kit is between 15.6pg/ml-1000pg/ml as stated in the datasheets.

Boster Scientific Support

Answered: 2016-11-09

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