Anti-Golgi phosphoprotein 3 GOLPH3 Antibody

Product Name

Anti-Golgi phosphoprotein 3 GOLPH3 Antibody

View all GOLPH3 Antibodies

SKU/Catalog Number

A01333

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Golgi phosphoprotein 3 GOLPH3 Antibody (Catalog # A01333). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

GOLPH3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Golgi phosphoprotein 3 GOLPH3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01333)

Host

Rabbit

Contents

GOLPH3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

GOLPH3 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human GOLPH3. The immunogen is located within the first 50 amino acids of GOLPH3.

Reactive Species

A01333 is reactive to GOLPH3 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

33.8 kDa

Background of GOLPH3

GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signalling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signalling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01333 is guaranteed for ELISA, IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

GOLPH3 antibody can be used for detection of GOLPH3 by Western blot at 0.5 - 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information GOLPH3

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