Anti-GOLPH2 GOLM1 Antibody

Product Name

Anti-GOLPH2 GOLM1 Antibody

View all GOLM1 Antibodies

SKU/Catalog Number

A02975

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-GOLPH2 GOLM1 Antibody (Catalog # A02975). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

GOLPH2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-GOLPH2 GOLM1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02975)

Host

Rabbit

Contents

GOLPH2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

GOLPH2 antibody was raised against an 18 amino acid synthetic peptide from near the center of human GOLPH2. The immunogen is located within amino acids 130 - 180 of GOLPH2.

Reactive Species

A02975 is reactive to GOLM1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

45.3 kDa

Background of GOLM1

GOLPH2, also known as GOLM1, is a Golgi phosphoprotein that has a short cytoplasmic N-terminal domain, a membrane spanning region, and a longer C-terminal domain. It was initially identified as a possible marker for Alzheimer's disease, although later studies have demonstrated that the GOLPH2 gene does not contribute to risk of this disease. GOLPH2 expression has been reported higher in prostate cancer tissues compared to normal prostate tissue, suggesting that GOLPH2 can be used as an additional positive marker for tissue-based diagnosis of prostate cancer. It has been suggested that GOLPH2 expression in hepatocellular carcinomas (HCCs) and serum may also serve as tumor markers for HCCs.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02975 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

GOLPH2 antibody can be used for detection of GOLPH2 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information GOLM1

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