Anti-GPR2/CCR10 Antibody

Product Name

Anti-GPR2/CCR10 Antibody

View all CCR10/GPR2 Antibodies

SKU/Catalog Number

PA1491

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GPR2/CCR10 Antibody catalog # PA1491. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GPR2/CCR10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1491)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human GPR2/CCR10, different from the related rat and mouse sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1491 is reactive to CCR10 in Human, Mouse, Rat

Applications

PA1491 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

20 kDa

Calculated molecular weight

38.416kDa

Background of CCR10/GPR2

CCR10, C-C chemokine receptor type 10 is a protein that in humans is encoded by the CCR10 gene. CCR10 is constitutively expressed in skin melanocytes, fibroblasts, and microvascular endothelial cells, and that it is upregulated by IL1B and TNF. Expression was also detected in T cells, Langerhans cells, and peripheral blood mononuclear cells but not in dendritic cells.By fluorescence in situ hybridization, the GPR2 gene is mapped to 17q21.1-q21.3. CCR10 is the receptor for CCL27. CCR10-CCL27 interactions are involved in T cell-mediated skin inflammation. expression of CCL27 in normal keratinocytes and its strong upregulation in skin lesions of atopic dermatitis, contact dermatitis, and psoriasis patients. CCR10+ T lymphocytes were detected in lesional but not normal skin of these patients. Flow cytometric analysis showed that CCL27 binds extracellular matrix components and dermal microvascular endothelial cells and fibroblasts and mediates adhesion and transendothelial migration of CCR10+ circulating leukocytes. CCR10 is predominantly expressed on CD4+CLA+ (cutaneous lymphocyte antigen), rather than CD8+, circulating T cells. RT-PCR, confocal microscopy, and ELISA analysis indicated that keratinocytes exposed to TNF or IL1B but not to IL4 or IFNG in vitro express increased CCL27.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

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Anti-GPR2/CCR10 antibody, PA1491, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: SW620 Cell Lysate
Lane 3: A549 Cell Lysate
Lane 4: MM231 Cell Lysate
Lane 5: SMMC Cell Lysate

Gene/Protein Information For CCR10 (Source: Uniprot.org, NCBI)

Alternative Names

CC chemokine receptor 10; C-C chemokine receptor type 10; C-C CKR-10; CC-CKR-10; CCR10; CCR-10; chemokine (C-C motif) receptor 10; G protein-coupled receptor 2; GPR2; GPR2G-protein coupled receptor 2 CCR10 GPR2 C-C motif chemokine receptor 10 C-C chemokine receptor type 10|C-C CKR-10|CC chemokine receptor 10|CC-CKR-10|CCR-10|G-protein coupled receptor 2|chemokine (C-C motif) receptor 10

For more info on CCR10, check out the CCR10 Infographic

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In this infographic, you will see the following information for CCR10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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