Anti-GRB2 Antibody Picoband™

Product Name

Anti-GRB2 Antibody Picoband™

View all GRB2 Antibodies

SKU/Catalog Number

A00351-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GRB2 Antibody Picoband™ catalog # A00351-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-GRB2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00351-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human GRB2 recombinant protein (Position: M1-V217).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A00351-1 is reactive to GRB2 in Human

Applications

A00351-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

25 kDa

Calculated molecular weight

25.206kDa

Background of GRB2

GRB2, Growth factor receptor-bound protein 2, is an adaptor protein involved in signal transduction/cell communication. The GRB2 gene encodes a protein that has homology to noncatalytic regions of the SRC oncogene product. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. In humans, the GRB2 protein is encoded by the GRB2 gene. Grb2 is widely expressed and is essential for multiple cellular functions. The protein encoded by this gene binds receptors such as the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the sem-5 gene of Caenorhabditis elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml/ml, Human
Flow Cytometry, 1-3 μg/ml/1x10^6 cells, Human
Direct ELISA, 0.1-0.5 μg/ml/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of GRB2 using anti-GRB2 antibody (A00351-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human HL-60 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRB2 antigen affinity purified polyclonal antibody (Catalog # A00351-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRB2 at approximately 25 kDa. The expected band size for GRB2 is at 25 kDa.

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Figure 2. Flow Cytometry analysis of PC-3 cells using anti-GRB2 antibody (A00351-1).
Overlay histogram showing PC-3 cells stained with A00351-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRB2 Antibody (A00351-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For GRB2 (Source: Uniprot.org, NCBI)

Alternative Names

abundant SRC homology; Adapter protein GRB2; ASH; EGFRBP-GRB2; epidermal growth factor receptor-binding protein GRB2; GRB2; Grb3-3; growth factor receptor-bound protein 2; growth factor receptor-bound protein 3; HT027; MST084; MSTP084; NCKAP2; Protein Ash; SH2/SH3 adapter GRB2 GRB2 ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2 growth factor receptor bound protein 2 growth factor receptor-bound protein 2|HT027|SH2/SH3 adapter GRB2|abundant SRC homology|epidermal growth factor receptor-binding protein GRB2|epididymis secretory sperm binding protein|growth factor receptor-bound protein 3|protein Ash

For more info on GRB2, check out the GRB2 Infographic

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In this infographic, you will see the following information for GRB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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