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- Table of Contents
HSPA5 antibody (PB9640) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 8 Publications
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IHC analysis of HSPA5 using anti-HSPA5 antibody (PB9640).
HSPA5 was detected in a paraffin-embedded section of human cerebral cortex tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSPA5 Antibody (PB9640) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
HSPA5 was detected in a paraffin-embedded section of human cerebral cortex tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSPA5 Antibody (PB9640) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-GRP78 BiP/HSPA5 Antibody Picoband®
HSPA5 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
HSPA5 antibody was positively detected by WB in:
human U251 whole cell, human HepG2 whole cell, human K562 whole cell, human A431 whole cell, rat testis tissue, rat liver tissue, mouse testis tissue, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
HSPA5 antibody was positively detected by IHC in:
human cerebral cortex tissue, mouse testis tissue, rat testis tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
HSPA5 antibody was positively detected by ICC/IF in:
Hela cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-GRP78 BiP/HSPA5 Antibody Picoband®
View all HSPA5 Antibodies
View all HSPA5 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human GRP78 BiP, identical to the related mouse and rat sequences.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
78 kDa
Calculated Molecular Weight
72.3 kDa
Background of HSPA5
HSPA5 (heat shock 70kDa protein 5), also known as glucose-regulated protein, 78kD (GRP78) or BiP, is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum. BiP is also an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by the proteasome. The HSPA5 gene is mapped on 9q33.3. Shen et al. (2002) concluded that HSPA5 retains ATF6 in the ER by inhibiting its Golgi localization signals and that dissociation of HSPA5 during ER stress allows ATF6 to be transported to the Golgi. The findings of Shen et al. (2002) demonstrated that HSPA5 is a key element in sensing the folding capacity within the ER.
Cite This Product
Anti-GRP78 BiP/HSPA5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9640)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Zebrafish
82%
Moderate Homology
Drosophila
48%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for HSPA5 antibody
Immunohistochemistry (IHC) protocol for HSPA5 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for HSPA5 antibody
Publications
Application:
Species:
Cadmium-Induced Kidney Apoptosis Based on the IRE1α-XBP1 Signaling Pathway and the Protective Effect of Quercetin
Published: 2025-02-10
Journal: Toxics
Application: WB
Impact Factor: 4.10
Species: Rat
PMID: 39997944
BHBA attenuates endoplasmic reticulum stress-dependent neuroinflammation via the gut–brain axis in a mouse model of heat stress
Published: 2024-07-07
Journal: CNS Neuroscience & Therapeutics
Application: WB
Impact Factor: 4.80
Species: Mouse
PMID: 38973202
Ketogenic diet attenuates cognitive dysfunctions induced by hypoglycemia via inhibiting endoplasmic reticulum stress-dependent pathways
Published: 2023-12-21
Journal: Food & Function
Application: WB
Impact Factor: 6.10
Species: Mouse
PMID: 38197246
Expression of ER stress markers (GRP78 and PERK) in experimental nephrotoxicity induced by cisplatin and gentamicin: roles of inflammatory response and oxidative stress
Published: 2022-12-09
Journal: NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
Application: IHC
Impact Factor: 3.20
Species: Rat
PMID: 36482225
ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes
Published: 2020-11-09
Journal: MEDIATORS OF INFLAMMATION
Application: WB
Impact Factor: 3.76
Species: Human
PMID: 33223956
miR-9-5p attenuates ischemic stroke through targeting ERMP1-mediated endoplasmic reticulum stress
Published: 2019-09-07
Journal: ACTA HISTOCHEMICA
Application: WB
Impact Factor: 1.72
Species: Human,Rat
PMID: 31500865
Local Delivery of β-Elemene Improves Locomotor Functional Recovery by Alleviating Endoplasmic Reticulum Stress and Reducing Neuronal Apoptosis in Rats with Spinal Cord Injury
Published: 2018-08-30
Journal: CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
Application: WB
Impact Factor: 5.50
Species: Rat
PMID: 30165357
Excessive fluoride induces endoplasmic reticulum stress and interferes enamel proteinases secretion
Published: 2011-05-27
Journal: ENVIRONMENTAL TOXICOLOGY
Application: IF
Impact Factor: 1.93
Species: Mouse
PMID: 21626649
Review
Have you used Anti-GRP78 BiP/HSPA5 Antibody Picoband®?
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1 Review For Anti-GRP78 BiP/HSPA5 Antibody Picoband®
Accurate localization and good imaging quality
| SKU | PB9640 |
|---|---|
| Application | Immunofluorenscence |
| Sample | Mouse 4T1 cell climbing slides |
| Sample Processing Description | Mouse 4T1 cells fixed with 4% paraformaldehyde for 15 minutes |
| Primary Antibody | Anti-GRP78 antibody |
| Primary Incubation | 1:400, overnight at 4 ℃ |
| Blocking Agent | Goat serum |
| Secondary Antibody | DyLight 550-conjugated goat anti-rabbit antibody. |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Detection | Laser confocal microscopy |
| Results Summary | The ordering and delivery cycle of the antibodies is short — I was able to receive the products within a week, which saved a lot of time. The pre-sales and after-sales services are convenient and efficient. Since my background is in chemistry, I consulted Boster’s technical staff about many details of biological experiments. The technical specialists were patient and helpful in their analysis and explanations, which allowed me to avoid many detours during the actual experimental procedures. Most importantly, these antibodies offer a high cost-performance ratio, with good binding performance, excellent imaging results, and a high experimental success rate, providing a solid foundation for conducting related experiments. |
Yanhua Li，Shandong Normal University
Verified customer
Submitted 2025-10-17
Product Promise
We promise Anti-GRP78 BiP/HSPA5 Antibody Picoband® performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9640
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 24, 2026