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Product Info Summary
| SKU: | A00827-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GTP-binding protein Rheb RHEB Antibody
SKU/Catalog Number
A00827-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-GTP-binding protein Rheb RHEB Antibody catalog # A00827-2. Tested in WB,ICC,IF,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GTP-binding protein Rheb RHEB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00827-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human PKC delta around the phosphorylation site of Y64.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00827-2 is reactive to RHEB in Human, Mouse
Applications
A00827-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
20.497kDa
Background of RHEB
Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12.
Arja Lamberg, J. Biol. Chem., May 1996; 271: 11988.
GW Conrad, J. Cell Biol., Mar 1980; 84: 501.
DR Keene, J. Cell Biol., Nov 1987; 105: 2393.
HM Wang, J. Histochem. Cytochem., Nov 1980; 28: 1215.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
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Figure 2. Immunocytochemistry staining of RHEB using Anti-GTP-binding protein Rheb RHEB Antibody (A00827-2).
ICC staining RHEB in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
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Figure 1. Western blotting validation for Anti-GTP-binding protein Rheb RHEB Antibody A00827-2
Western blot analysis of RHEB on mouse placenta tissue lysate using anti-RHEB antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For RHEB (Source: Uniprot.org, NCBI)
Gene Name
RHEB
Full Name
GTP-binding protein Rheb
Weight
20.497kDa
Superfamily
small GTPase superfamily
Alternative Names
GTP-binding protein Rheb; MGC111559; Ras homolog enriched in brainRHEB2Ras homolog enriched in brain 2; Rheb; Rheb2 RHEB RHEB2 Ras homolog, mTORC1 binding GTP-binding protein Rheb|Ras homolog enriched in brain 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RHEB, check out the RHEB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RHEB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GTP-binding protein Rheb RHEB Antibody (A00827-2)
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No publications found for A00827-2
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