Anti-GTP-binding protein Rheb Rheb Antibody

Product Name

Anti-GTP-binding protein Rheb Rheb Antibody

View all RHEB Antibodies

SKU/Catalog Number

A00827-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-GTP-binding protein Rheb Rheb Antibody (Catalog # A00827-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Rheb antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-GTP-binding protein Rheb Rheb Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00827-1)

Host

Rabbit

Contents

Rheb Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Rheb antibody was raised against a 15 amino acid synthetic peptide from the middle region of human Rheb. The immunogen is located within amino acids 40 - 90 of Rheb.

Reactive Species

A00827-1 is reactive to RHEB in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

20.5 kDa

Background of RHEB

Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway. Biochemical and genetic studies demonstrate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway. TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00827-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Rheb antibody can be used for the detection of Rheb by Western blot at 1 to 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information RHEB

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