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Product Info Summary
| SKU: | M00325-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-HDAC2/Histone Deacetylase 2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00325-1
BM3967 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HDAC2/Histone Deacetylase 2 Rabbit Monoclonal Antibody catalog # M00325-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HDAC2/Histone Deacetylase 2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00325-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAE-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HDAC2
Reactive Species
M00325-1 is reactive to HDAC2 in Human, Mouse, Rat
Observed Molecular Weight
60 kDa
Calculated molecular weight
55.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00325-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:30
FC 1:50
Positive Control
WB: human HL-60 whole cell, human THP-1 whole cel, human U937 whole cell, human Caco-2 whole cell, human U2OS whole cell, human A431 whole cell, human HepG2 whole cell
IHC: human breast cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of HDAC2 using anti-HDAC2 antibody (M00325-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human U937 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: human U2OS whole cell lysates,
Lane 6: human A431 whole cell lysates,
Lane 7: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC2 antigen affinity purified monoclonal antibody (M00325-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HDAC2 at approximately 60 kDa. The expected band size for HDAC2 is at 55 kDa.
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IHC analysis of HDAC2 using anti-HDAC2 antibody (M00325-1).
HDAC2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HDAC2 Antibody (M00325-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-HDAC2/Histone Deacetylase 2 Rabbit Monoclonal Antibody (M00325-1)
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