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Product Info Summary
| SKU: | M02237 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Hemopexin HPX Monoclonal Antibody
SKU/Catalog Number
M02237
BM5363 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Hemopexin HPX Monoclonal Antibody catalog # M02237. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hemopexin HPX Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02237)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADAD-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Hemopexin Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
Reactive Species
M02237 is reactive to HPX in Human
Observed Molecular Weight
70 kDa
Calculated molecular weight
51.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02237 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: human HepG2 whole cell, human Caco-2 whole cell, human hepatocellular carcinoma tumor tissue (HCCT)
Validation Images & Assay Conditions
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Immunohistochemistry of FGA, F2, CFH, PIPOX, ITIH4, GNMT, MAT1A, MTHFD1, HPX, CTH, CFHR3, ENNP1, and NAT2 in clinical GBC and control specimens.
Index in PubMed under a CC BY license. PMID: 33718182
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Western blot analysis of Hemopexin/HPX using anti-Hemopexin/HPX antibody (M02237).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human hepatocellular carcinoma tumor tissue (HCCT) lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hemopexin/HPX antigen affinity purified monoclonal antibody (M02237) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Hemopexin/HPX at approximately 70 kDa. The expected band size for Hemopexin/HPX is at 52 kDa.
Specific Publications For Anti-Hemopexin HPX Monoclonal Antibody (M02237)
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Customer Q&As
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4 Customer Q&As for Anti-Hemopexin HPX Monoclonal Antibody
Question
Does anti-Hemopexin Monoclonal antibody M02237 work for IP with right lobe of liver?
E. Jackson
Verified customer
Asked: 2020-03-26
Answer
According to the expression profile of right lobe of liver, HPX is highly expressed in right lobe of liver. So, it is likely that anti-Hemopexin Monoclonal antibody M02237 will work for IP with right lobe of liver.
Boster Scientific Support
Answered: 2020-03-26
Question
We are currently using anti-Hemopexin Monoclonal antibody M02237 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
The anti-Hemopexin Monoclonal antibody (M02237) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-14
Question
I see that the anti-Hemopexin Monoclonal antibody M02237 works with IP, what is the protocol used to produce the result images on the product page?
A. Krishna
Verified customer
Asked: 2016-01-01
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-01-01
Question
Is a blocking peptide available for product anti-Hemopexin Monoclonal antibody (M02237)?
H. Brown
Verified customer
Asked: 2015-02-27
Answer
We do provide the blocking peptide for product anti-Hemopexin Monoclonal antibody (M02237). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2015-02-27
