Product Info Summary
SKU: | PA1787-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-HEXA Antibody
View all Hexosaminidase A/HEXA Antibodies
SKU/Catalog Number
PA1787-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HEXA Antibody catalog # PA1787-2. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HEXA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1787-2)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human HEXA, different from the related mouse sequence by three amino acids, and from the related rat sequences by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1787-2 is reactive to HEXA in Human
Applications
PA1787-2 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
57 kDa
Calculated molecular weight
60.703kDa
Background of Hexosaminidase A/HEXA
HEXA (hexosaminidase A (alpha polypeptide)) is an enzyme that in humans is encoded by the HEXA gene. Hexosaminidase A and the cofactor GM2 activator protein catalyze the degradation of the GM2 gangliosides and other molecules containing terminal N-acetyl hexosamines The HEXA gene encodes the alpha subunit of hexosaminidase A, a lysosomal enzyme involved in the breakdown of gangliosides. The HEXA gene is mapped on 15q23. Even though the alpha and beta subunits of hexosaminidase A can both cleave GalNAc residues, only the alpha subunit is able to hydrolyze GM2 gangliosides. The alpha subunit contains a key residue, Arg-424, which is essential for binding the N-acetyl-neuramanic residue of GM2 gangliosides. Chimeric constructs were expressed in HeLa cells and selected constructs were produced in the baculovirus expression system to determine their ability to degrade GM2 ganglioside in the presence of GM2 activator protein. Their results allowed them to define 2 noncontiguous sequences in the alpha subunit (amino acids 1-191 and 403-529) which, when substituted into analogous positions in the beta subunit, conferred activity against the sulfated substrate.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of HEXA using anti-HEXA antibody (PA1787-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEXA antigen affinity purified polyclonal antibody (Catalog # PA1787-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HEXA at approximately 57 kDa. The expected band size for HEXA is at 60 kDa.
Protein Target Info & Infographic
Gene/Protein Information For HEXA (Source: Uniprot.org, NCBI)
Gene Name
HEXA
Full Name
Beta-hexosaminidase subunit alpha
Weight
60.703kDa
Superfamily
glycosyl hydrolase 20 family
Alternative Names
beta-hexosaminidase subunit alpha; Beta-N-acetylhexosaminidase subunit alpha; EC 3.2.1; EC 3.2.1.52; HEXA; hexosaminidase A (alpha polypeptide); Hexosaminidase A; Hexosaminidase subunit A; MGC99608; N-acetyl-beta-glucosaminidase subunit alpha; TSD HEXA TSD hexosaminidase subunit alpha beta-hexosaminidase subunit alpha|N-acetyl-beta-glucosaminidase subunit alpha|beta-N-acetylhexosaminidase subunit alpha|hexosaminidase A (alpha polypeptide)|hexosaminidase subunit A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HEXA, check out the HEXA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HEXA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HEXA Antibody (PA1787-2)
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No publications found for PA1787-2
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-HEXA Antibody
Question
Will anti-HEXA antibody PA1787-2 work for WB with thalamus?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
According to the expression profile of thalamus, HEXA is highly expressed in thalamus. So, it is likely that anti-HEXA antibody PA1787-2 will work for WB with thalamus.
Boster Scientific Support
Answered: 2020-05-05
Question
Does anti-HEXA antibody PA1787-2 work on zebrafish WB with thalamus?
Verified Customer
Verified customer
Asked: 2020-04-15
Answer
Our lab technicians have not tested anti-HEXA antibody PA1787-2 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-HEXA antibody PA1787-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish thalamus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-15
Question
Would PA1787-2 anti-HEXA antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
You can see on the product datasheet, PA1787-2 anti-HEXA antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-03
Question
Is a blocking peptide available for product anti-HEXA antibody (PA1787-2)?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
We do provide the blocking peptide for product anti-HEXA antibody (PA1787-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-26
Question
I was wanting to use your anti-HEXA antibody for WB for human thalamus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human thalamus identification?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
You can see on the product datasheet, PA1787-2 anti-HEXA antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human thalamus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-27
Question
We are currently using anti-HEXA antibody PA1787-2 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
A. Singh
Verified customer
Asked: 2019-04-29
Answer
The anti-HEXA antibody (PA1787-2) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-29