Anti-HIF-alpha HIF1A Monoclonal Antibody

Product Name

Anti-HIF-alpha HIF1A Monoclonal Antibody

View all HIF-1 alpha Antibodies

SKU/Catalog Number

M00013-2

Size

100ul

Form

Liquid

Description

Boster Bio Anti-HIF-alpha HIF1A Monoclonal Antibody catalog # M00013-2. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HIF-alpha HIF1A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00013-2)

Host

Mouse

Contents

Ascitic fluid containing 0.03% sodium azide.

Clonality

Monoclonal

Clone Number

1A3

Isotype

IgG

Immunogen

Purified recombinant fragment of human HIF-1alpha expressed in E. Coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00013-2 is reactive to HIF1A in Human, Monkey, Mouse

Applications

M00013-2 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

92.67kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
ELISA 1:10000

Validation Images & Assay Conditions

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Figure 4. ELISA validation of HIF1A using Anti-HIF-alpha HIF1A Monoclonal Antibody (M00013-2).

ELISA analysis of HIF-1alpha antibody.

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Figure 3. Immunofluorescent staining data of HIF1A using Anti-HIF-alpha HIF1A Monoclonal Antibody (M00013-2).

Immunofluorescence (IF) analysis of HeLa cells using HIF-1alpha Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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Figure 2. Immunohistochemistry validation of HIF1A using Anti-HIF-alpha HIF1A Monoclonal Antibody (M00013-2).

Immunohistochemistry (IHC) analysis of paraffin-embedded liver cancer tissues (left) and Lung Cancer Tissues (right) with DAB staining using HIF-1alpha Monoclonal Antibody.
For more protocol information of IHC

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Figure 1. Western blotting validation for Anti-HIF-alpha HIF1A Monoclonal Antibody M00013-2

Western Blot (WB) analysis using HIF-1alpha Monoclonal Antibody against Cos7 (1)

Gene/Protein Information For HIF1A (Source: Uniprot.org, NCBI)

Alternative Names

AINT; HIF-1 alpha; HIF1A; ARNT interacting protein; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; BHLHE78; Class E basic helix-loop-helix protein 78; H1alpha67; HIF 1A; HIF1 alpha; HIF-1 alpha; HIF1; HIF1A; HIF-1a; HIF-1alpha; HIF-1-alpha; HIF1-alpha; hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1-alpha; Member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8 HIF1A HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78 hypoxia inducible factor 1 subunit alpha hypoxia-inducible factor 1-alpha|ARNT interacting protein|PAS domain-containing protein 8|basic-helix-loop-helix-PAS protein MOP1|class E basic helix-loop-helix protein 78|hypoxia inducible factor 1 alpha subunit|hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)|hypoxia-inducible factor1alpha|member of PAS protein 1|member of PAS superfamily 1

For more info on HIF1A, check out the HIF1A Infographic

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In this infographic, you will see the following information for HIF1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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