Product Info Summary
SKU: | A01843 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Histone deacetylase 8 HDAC8 Antibody
View all Histone Deacetylase 8/HDAC8 Antibodies
SKU/Catalog Number
A01843
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Histone deacetylase 8 HDAC8 Antibody catalog # A01843. Tested in WB,ICC/IF,IHC,IP,Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone deacetylase 8 HDAC8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01843)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human ApoL1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01843 is reactive to HDAC8 in Human
Applications
A01843 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
41.758kDa
Background of Histone Deacetylase 8/HDAC8
Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2 and GRIK2) and serotonin (HTR2C), GABA receptor (GABRA3) and potassium voltage-gated channel (KCNA1). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alter their functional activities. Edits GRIA2 at both the Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently. Can exert a proviral effect towards human immunodeficiency virus type 1 (HIV-1) and enhances its replication via both an editing-dependent and editing-independent mechanism. The former involves editing of adenosines in the 5'UTR while the latter occurs via suppression of EIF2AK2/PKR activation and function. Can inhibit cell proliferation and migration and can stimulate exocytosis.
Mittaz L., Genomics 41:210-217(1997).
Lai F., Mol. Cell. Biol. 17:2413-2424(1997).
Villard L., Somat. Cell Mol. Genet. 23:135-145(1997).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:2,000
ICC: 1:100-1:500
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of HDAC8 using Anti-Histone deacetylase 8 HDAC8 Antibody (A01843).
ICC staining HDAC8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-Histone deacetylase 8 HDAC8 Antibody A01843
Western blot analysis of HDAC8 on different lysates using anti-HDAC8 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For HDAC8 (Source: Uniprot.org, NCBI)
Gene Name
HDAC8
Full Name
Histone deacetylase 8
Weight
41.758kDa
Superfamily
histone deacetylase family
Alternative Names
EC 3.5.1.98; HD8; HDAC8; HDACL1; Histone Deacetylase 8; histone deacetylase-like 1; RPD3 HDAC8 CDA07, CDLS5, HD8, HDACL1, KDAC8, MRXS6, RPD3, WTS histone deacetylase 8 histone deacetylase 8|histone deacetylase-like 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HDAC8, check out the HDAC8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HDAC8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Histone deacetylase 8 HDAC8 Antibody (A01843)
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No publications found for A01843
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