Product Info Summary
SKU: | M12477-15 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ChIP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Histone H3 (acetyl K27) Rabbit Monoclonal Antibody
SKU/Catalog Number
M12477-15
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Histone H3 (acetyl K27) Rabbit Monoclonal Antibody catalog # M12477-15. Tested in WB, IHC, ICC/IF, ChIP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H3 (acetyl K27) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12477-15)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
26H47
Isotype
IgG
Immunogen
A synthesized peptide derived from Histone H3 (acetyl K27)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M12477-15 is reactive to in Human, Mouse, Rat
Applications
M12477-15 is guaranteed for ChIP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
17 kDa
Calculated molecular weight
15 kDa
Background of
Putative transcription factor involved in pancreas development and function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
ChIP 1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Histone H3 using anti-Histone H3 antibody (M12477-15).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human CACO-2 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse RAW264.7 whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Histone H3 antigen affinity purified monoclonal antibody (Catalog # M12477-15) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Histone H3 at approximately 15 kDa. The expected band size for Histone H3 is at 15 kDa.
Specific Publications For Anti-Histone H3 (acetyl K27) Rabbit Monoclonal Antibody (M12477-15)
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