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Product Info Summary
| SKU: | M00194-4 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-HLA-A Antibody (Monoclonal, 34H66)
SKU/Catalog Number
M00194-4
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-HLA-A Antibody (Monoclonal, 34H66) catalog # M00194-4. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HLA-A Antibody (Monoclonal, 34H66) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00194-4)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
34H66
Immunogen
Recombinant protein within human HLA-B aa 112-307.
Reactive Species
M00194-4 is reactive to HLA-A in Human, Mouse, Rat
Observed Molecular Weight
41,44 kDa
Calculated molecular weight
40.8 kDa
Background of HLA-A
HLA-A, namely HLA A, is a group of human leukocyte antigens (HLA) that are coded for by the HLA-A locus, which is located at human chromosome 6p21.3. HLA is simply the major histocompatibility complex (MHC) specific to humans. HLA-A is one of three major types of human MHC class I cell surface receptors. The others are HLA-B and HLA-C. And it is critical to the cytotoxic t-cell controlled immune response to viruses and other intracellular pathogens. Because each HLA-A gene has a high affinity for slightly different peptides, certain HLA-As are associated with increased risk, more rapid progression, and/or increased severity of many diseases. For similar reasons, HLA-A matching is essential to successful tissue transplants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00194-4 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-200
Immunocytochemistry/Immunofluorescence, 1:50-200
Positive Control
WB: human A549 whole cell, human 293T whole cell, human Hela whole cell, human HepG2 whole cell lysates, human A431 whole cell lysates, human HEL whole cell lysates, rat lung tissue lysates, mouse lung tissue lysates.
Validation Images & Assay Conditions
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Western blot analysis of HLA-A using anti-HLA-A antibody (M00194-4).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human A431 whole cell lysates,
Lane 6: human HEL whole cell lysates,
Lane 7: rat lung tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA-A antigen affinity purified monoclonal antibody (M00194-4) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HLA-A at approximately 41,44 kDa. The expected band size for HLA-A is at 41 kDa.
Specific Publications For Anti-HLA-A Antibody (Monoclonal, 34H66) (M00194-4)
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