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Product Info Summary
| SKU: | M02651 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-HMGB2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02651
BM5242 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HMGB2 Rabbit Monoclonal Antibody catalog # M02651. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HMGB2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02651)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACCF-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HMGB2
Reactive Species
M02651 is reactive to HMGB2 in Human, Mouse, Rat
Observed Molecular Weight
24 kDa
Calculated molecular weight
24.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02651 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: HeLa cell, PC-12 cell
Validation Images & Assay Conditions
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Western blot analysis of HMGB2 expression in (1) HeLa cell lysate; (2) PC-12 cell lysate.
Specific Publications For Anti-HMGB2 Rabbit Monoclonal Antibody (M02651)
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Customer Q&As
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4 Customer Q&As for Anti-HMGB2 Rabbit Monoclonal Antibody
Question
What sample target on Western Blot application was tested with M02651?
Verified customer
Asked: 2020-01-03
Answer
Western blot analysis of HMGB2 expression in (1) HeLa cell lysate; (2) PC-12 cell lysate.
Boster Scientific Support
Answered: 2020-01-16
Question
Will M02651 anti-HMGB2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
You can see on the product datasheet, M02651 anti-HMGB2 Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-03
Question
I see that the anti-HMGB2 Monoclonal antibody M02651 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-04-03
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-04-03
Question
Would anti-HMGB2 Monoclonal antibody M02651 work on feline IF with brain?
A. Roberts
Verified customer
Asked: 2016-06-01
Answer
Our lab technicians have not tested anti-HMGB2 Monoclonal antibody M02651 on feline. You can run a BLAST between feline and the immunogen sequence of anti-HMGB2 Monoclonal antibody M02651 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline brain in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-06-01
