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Product Info Summary
| SKU: | A05313-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-HMGCS1 Antibody (Center)
SKU/Catalog Number
A05313-1
Size
80 µl
Description
Boster Bio Anti-HMGCS1 Antibody (Center) (Catalog # A05313-1). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-HMGCS1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05313-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This HMGCS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-317 amino acids from the Central region of human HMGCS1.
Reactive Species
A05313-1 is reactive to HMGCS1 in Human, Mouse
Calculated molecular weight
57.3 kDa
Background of HMGCS1
HMGCS1 condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05313-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
FC: 1:10-1:50
Validation Images & Assay Conditions
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HMGCS1 Antibody (Center) western blot analysis in U87-MG cell line,mouse liver and rat liver lysates (35ug/lane).This demonstrates the HMGCS1 antibody detected the HMGCS1 protein (arrow).
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Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HMGCS1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Flow cytometric analysis of hela cells using HMGCS1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-HMGCS1 Antibody (Center) (A05313-1)
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