Anti-hnRNP A1 Rabbit Monoclonal Antibody

hnRNP A1 antibody

Boster Bio Anti-hnRNP A1 Rabbit Monoclonal Antibody catalog # M01476. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01476
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-hnRNP A1 Rabbit Monoclonal Antibody

View all hnRNP A1 Antibodies

SKU/Catalog Number

M01476

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-hnRNP A1 Rabbit Monoclonal Antibody catalog # M01476. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-hnRNP A1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01476)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ABEC-8

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human hnRNP A1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01476 is reactive to HNRNPA1 in Human, Mouse, Rat

Applications

M01476 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

59 kDa

Calculated molecular weight

38.747kDa

Background of hnRNP A1

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For HNRNPA1 (Source: Uniprot.org, NCBI)

Gene Name

HNRNPA1

Full Name

Heterogeneous nuclear ribonucleoprotein A1

Weight

38.747kDa

Alternative Names

Helix-destabilizing protein; heterogeneous nuclear ribonucleoprotein A1; heterogeneous nuclear ribonucleoprotein A1B protein; heterogeneous nuclear ribonucleoprotein B2 protein; heterogeneous nuclear ribonucleoprotein core protein A1; hnRNP A1; hnRNP core protein A1; hnRNPA1; hnRNP-A1; HNRPA1MGC102835; nuclear ribonucleoprotein particle A1 protein; single-strand DNA-binding protein UP1; Single-strand RNA-binding protein HNRNPA1 ALS19, ALS20, HNRPA1, HNRPA1L3, IBMPFD3, UP 1, hnRNP A1, hnRNP-A1 heterogeneous nuclear ribonucleoprotein A1 heterogeneous nuclear ribonucleoprotein A1|epididymis secretory sperm binding protein|helix-destabilizing protein|heterogeneous nuclear ribonucleoprotein A1B protein|heterogeneous nuclear ribonucleoprotein B2 protein|heterogeneous nuclear ribonucleoprotein core protein A1|hnRNP core protein A1-like 3|nuclear ribonucleoprotein particle A1 protein|putative heterogeneous nuclear ribonucleoprotein A1-like 3|single-strand DNA-binding protein UP1|single-strand RNA-binding protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HNRNPA1, check out the HNRNPA1 Infographic

HNRNPA1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HNRNPA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-hnRNP A1 Rabbit Monoclonal Antibody

Question

For M01476 Anti-HnRNP A1 Rabbit Monoclonal Antibody, what's the sequence of the immunogen peptide?

Verified customer

Asked: 2022-06-15

Answer

Thank you for reaching out to us. Please see the following immunogen sequence. DFGNYNNQSSNFGP

Boster Scientific Support

Answered: 2022-06-16

Question

Can you help my question with product M01476, anti-hnRNP A1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-29

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01476 anti-hnRNP A1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-29

Question

Would M01476 anti-hnRNP A1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-12-20

Answer

As indicated on the product datasheet, M01476 anti-hnRNP A1 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-12-20

Question

Is a blocking peptide available for product anti-hnRNP A1 Rabbit Monoclonal antibody (M01476)?

Verified Customer

Verified customer

Asked: 2018-03-16

Answer

We do provide the blocking peptide for product anti-hnRNP A1 Rabbit Monoclonal antibody (M01476). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-03-16

Question

I was wanting to use your anti-hnRNP A1 Rabbit Monoclonal antibody for WB for rat cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat cervix carcinoma identification?

C. Patel

Verified customer

Asked: 2015-02-10

Answer

It shows on the product datasheet, M01476 anti-hnRNP A1 Rabbit Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-02-10

Order DetailsPrice
M01476

100μL

$370
M01476-30ul

30μL sample

$99
M01476-carrier-free

Carrier Free

$370

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Free Secondary Antibody

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M01476
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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