Anti-HPRT/HPRT1 Antibody Picoband™

Product Name

Anti-HPRT/HPRT1 Antibody Picoband™

View all HPRT Antibodies

SKU/Catalog Number

PB10071

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HPRT/HPRT1 Antibody Picoband™ catalog # PB10071. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HPRT/HPRT1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10071)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human HPRT recombinant protein (Position: A2-S110). Human HPRT shares 98.1% and 96.3% amino acid (aa) sequence identity with mouse and rat HPRT, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB10071 is reactive to HPRT1 in Human, Mouse, Rat

Applications

PB10071 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

25 kDa

Calculated molecular weight

24.579kDa

Background of HPRT

Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) is an enzyme encoded in humans by the HPRT1 gene. The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of HPRT/HPRT1 using anti-HPRT/HPRT1 antibody (PB10071).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Colo320 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HPRT/HPRT1 antigen affinity purified polyclonal antibody (Catalog # PB10071) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HPRT/HPRT1 at approximately 25 kDa. The expected band size for HPRT/HPRT1 is at 25 kDa.

Gene/Protein Information For HPRT1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.4.2.8; HGPRT; HGPRT1; HGPRTHPRTHGPRTase; HPRT; HPRT1; hypoxanthine phosphoribosyltransferase 1; hypoxanthine-guanine phosphoribosyltransferase HPRT1 HGPRT, HPRT hypoxanthine phosphoribosyltransferase 1 hypoxanthine-guanine phosphoribosyltransferase|HGPRTase|hypoxanthine-guanine phosphoribosyltransferase 1|testicular tissue protein Li 89

For more info on HPRT1, check out the HPRT1 Infographic

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In this infographic, you will see the following information for HPRT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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