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Product Info Summary
| SKU: | A07397-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-HSD17B12 Antibody (Center)
SKU/Catalog Number
A07397-1
Size
80 µl
Description
Boster Bio Anti-HSD17B12 Antibody (Center) (Catalog # A07397-1). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-HSD17B12 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07397-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This HSD17B12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-155 amino acids from the Central region of human HSD17B12.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07397-1 is reactive to HSD17B12 in Human
Applications
A07397-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
34.324kDa
Background of HSD17B12
The enzyme 17-beta hydroxysteroid dehydrogenase-12 (HSD17B12) uses NADPH to reduce 3-ketoacyl-CoA to 3-hydroxyacyl-CoA during the second step of fatty acid elongation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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HSD17B12 Antibody (Center) western blot analysis in A431,SiHa cell line lysates (35ug/lane).This demonstrates the HSD17B12 antibody detected the HSD17B12 protein (arrow).
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Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HSD17B12 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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HSD17B12 Antibody (Center) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For HSD17B12 (Source: Uniprot.org, NCBI)
Gene Name
HSD17B12
Full Name
Very-long-chain 3-oxoacyl-CoA reductase
Weight
34.324kDa
Superfamily
short-chain dehydrogenases/reductases (SDR) family
Alternative Names
17-beta-HSD 12; 17-beta-hydroxysteroid dehydrogenase 12; EC 1.1.1.62,3-ketoacyl-CoA reductase; EC 1.3.1.-; hydroxysteroid (17-beta) dehydrogenase 12,17beta-HSD type 12; KARestradiol 17-beta-dehydrogenase 12; SDR12C1; short chain dehydrogenase/reductase family 12C, member 1; steroid dehydrogenase homolog HSD17B12 KAR, SDR12C1 hydroxysteroid 17-beta dehydrogenase 12 very-long-chain 3-oxoacyl-CoA reductase|17-beta-HSD 12|17-beta-hydroxysteroid dehydrogenase 12|17beta-HSD type 12|3-ketoacyl-CoA reductase|estradiol 17-beta-dehydrogenase 12|short chain dehydrogenase/reductase family 12C member 1|steroid dehydrogenase homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSD17B12, check out the HSD17B12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSD17B12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSD17B12 Antibody (Center) (A07397-1)
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No publications found for A07397-1
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Customer Reviews
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