Anti-HSD3B2 Antibody

Product Name

Anti-HSD3B2 Antibody

View all Beta Hydroxysteroid Dehydrogenase Antibodies

SKU/Catalog Number

A02012

Size

100ul

Form

Liquid

Description

Boster Bio Anti-HSD3B2 Antibody catalog # A02012. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HSD3B2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02012)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human MEK-6 around the phosphorylation site of S207.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02012 is reactive to HSD3B2 in Human

Applications

A02012 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

42.052kDa

Background of Beta Hydroxysteroid Dehydrogenase

Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion.

Chicheportiche Y., J. Biol. Chem. 272:32401-32410(1997).
Marsters S.A., Curr. Biol. 8:525-528(1998).
Clark H.F., Genome Res. 13:2265-2270(2003).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-HSD3B2 Antibody A02012

Western blot (WB) analysis of HSD3B2 polyclonal antibody at 1:500 dilution
Lane1:HEK293T whole cell lysate
Lane2:Raw264.7 whole cell lysate
Lane3:H9C2 whole cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For HSD3B2 (Source: Uniprot.org, NCBI)

Alternative Names

3 beta-HSD type II; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II; 3-beta-HSD II; delta 5-delta 4-isomerase type II; HSD3B; HSDB; HSDB3B; hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2,3-beta-hydroxy-Delta(5)-steroid dehydrogenase; progesterone reductase; SDR11E2; short chain dehydrogenase/reductase family 11E, member 2,3-beta-hydroxy-5-ene steroid dehydrogenase HSD3B2 HSD3B, HSDB, SDR11E2 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2|3 beta-HSD type II|3 beta-hydroxysteroid dehydrogenase type II, delta 5-delta 4-isomerase type II, 3 beta-HSD type II|3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II|3-beta-HSD II|3-beta-HSD adrenal and gonadal type|3-beta-hydroxy-5-ene steroid dehydrogenase|3-beta-hydroxy-Delta(5)-steroid dehydrogenase|delta 5-delta 4-isomerase type II|progesterone reductase|short chain dehydrogenase/reductase family 11E, member 2

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