Product Info Summary
SKU: | A07981 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-HSP20 HSPB6 Antibody
View all HSP20/HSPB6 Antibodies
SKU/Catalog Number
A07981
Size
100ul
Form
Liquid
Description
Boster Bio Anti-HSP20 HSPB6 Antibody catalog # A07981. Tested in WB,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSP20 HSPB6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07981)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human COPZ1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07981 is reactive to HSPB6 in Human, Mouse, Rat
Applications
A07981 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
17.136kDa
Background of HSP20/HSPB6
The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors By similarity.The zeta subunit may be involved in regulating the coat assembly and, hence, the rate of biosynthetic protein transport due to its association-dissociation properties with the coatomer complex.
Futatsumori M., J. Biochem. 128:793-801(2000).
Lai C.-H., Genome Res. 10:703-713(2000).
Zhang Q.-H., Genome Res. 10:1546-1560(2000)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:2,000
IHC: 1:50-1:500
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of HSPB6 using Anti-HSP20 HSPB6 Antibody (A07981).
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Hsp20 antibody. Counter stained with hematoxylin.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-HSP20 HSPB6 Antibody A07981
Western blot analysis of Hsp20 on different lysates using anti-Hsp20 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For HSPB6 (Source: Uniprot.org, NCBI)
Gene Name
HSPB6
Full Name
Heat shock protein beta-6
Weight
17.136kDa
Superfamily
small heat shock protein (HSP20) family
Alternative Names
FLJ32389; Heat shock 20 kDa-like protein p20; heat shock protein beta-6; heat shock protein, alpha-crystallin-related, B6; HSP20; HSPB6 HSPB6 HEL55, Hsp20, PPP1R91 heat shock protein family B (small) member 6 heat shock protein beta-6|epididymis luminal protein 55|epididymis secretory sperm binding protein|heat shock 20 kDa-like protein p20|heat shock protein family B (small) member B6|heat shock protein, alpha-crystallin-related, B6|protein phosphatase 1, regulatory subunit 91
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSPB6, check out the HSPB6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSPB6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSP20 HSPB6 Antibody (A07981)
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No publications found for A07981
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