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Product Info Summary
| SKU: | M01692-3 |
|---|---|
| Size: | 200ug |
| Reactive Species: | Chicken, Dog, Hamster, Human, Mouse, Rabbit, Rat, Fish, Shark |
| Host: | Mouse |
| Application: | ELISA, IP, IF, IHC, ICC, WB, Antibody Microarray |
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Product info
Product Name
Anti-HSP90 Monoclonal Antibody
View all HSP90 beta Antibodies
SKU/Catalog Number
M01692-3
Size
200ug
Form
liquid
Description
Boster Bio Anti-HSP90 Monoclonal Antibody catalog # M01692-3. Tested in ELISA, IP, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSP90 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01692-3)
Host
Mouse
Contents
PBS pH7.2, 50% glycerol, 0.09% sodium azide
Clonality
Monoclonal
Clone Number
H9010
Isotype
IgG2a
Immunogen
Recombinant human HSP90beta
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects 90kDa. Detects HSP90 beta in all reactive species except in Chicken, where it detects both alpha and beta isoforms.
Reactive Species
M01692-3 is reactive to HSP90AB1 in Chicken, Dog, Hamster, Human, Mouse, Rabbit, Rat, Fish, Shark
Applications
M01692-3 is guaranteed for ELISA, IP, IF, IHC, ICC, WB, Antibody Microarray Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
83.264kDa
Background of HSP90 beta
HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms α and β, which share 85% sequence amino acid homology. The two isoforms of HSP90, are expressed in the cytosolic compartment (1). Despite the similarities, HSP90α exists predominantly as a homodimer while HSP90β exists mainly as a monomer (2). From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (3-6). Furthermore, HSP90 is highly conserved between species; having 60% and 78% amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively. HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite it's label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling (7-8). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase (5). When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (9). For more information visit our HSP90 Scientific Resource Guide at http://www.HSP90.ca.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB (1:2500), IHC (1:100); optimal dilutions for assays should be determined by the user.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC analysis of HSP90AB1 using anti-HSP90AB1 antibody (M01692-3).
HSP90AB1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HSP90AB1 Antibody (M01692-3) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 2. IHC analysis of HSP90AB1 using anti-HSP90AB1 antibody (M01692-3).
HSP90AB1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HSP90AB1 Antibody (M01692-3) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 3. Western blot analysis of HSP90AB1 using anti-HSP90AB1 antibody (M01692-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP90AB1 antigen affinity purified polyclonal antibody (Catalog # M01692-3) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for HSP90AB1.
Click image to see more details
Figure 4. IHC analysis of HSP90AB1 using anti-HSP90AB1 antibody (M01692-3).
HSP90AB1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HSP90AB1 Antibody (M01692-3) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of HSP90AB1 using anti-HSP90AB1 antibody (M01692-3).
HSP90AB1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HSP90AB1 Antibody (M01692-3) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of HSP90AB1 using anti-HSP90AB1 antibody (M01692-3).
HSP90AB1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HSP90AB1 Antibody (M01692-3) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 7. Western blot analysis of HSP90AB1 using anti-HSP90AB1 antibody (M01692-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP90AB1 antigen affinity purified polyclonal antibody (Catalog # M01692-3) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for HSP90AB1.
Click image to see more details
Figure 8. Western blot analysis of HSP90AB1 using anti-HSP90AB1 antibody (M01692-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP90AB1 antigen affinity purified polyclonal antibody (Catalog # M01692-3) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for HSP90AB1.
Protein Target Info & Infographic
Gene/Protein Information For HSP90AB1 (Source: Uniprot.org, NCBI)
Gene Name
HSP90AB1
Full Name
Heat shock protein HSP 90-beta
Weight
83.264kDa
Superfamily
heat shock protein 90 family
Alternative Names
D6S182; Heat shock 84 kDa; heat shock 90kD protein 1, beta; heat shock 90kDa protein 1, beta; heat shock protein 90kDa alpha (cytosolic), class B member 1; heat shock protein beta; heat shock protein HSP 90-beta; HSP 84; HSP84; HSP90 beta; HSP90AB1; HSP90B; HSP90-BETA; HSP90BHSP 90; HSPC2; HSPC2D6S182; HSPCB; HSPCBFLJ26984 HSP90AB1 D6S182, HSP84, HSP90B, HSPC2, HSPCB heat shock protein 90 alpha family class B member 1 heat shock protein HSP 90-beta|HSP90-beta|heat shock 84 kDa|heat shock 90kD protein 1, beta|heat shock protein 90 kDa|heat shock protein 90kDa alpha (cytosolic), class B member 1|heat shock protein 90kDa alpha family class B member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSP90AB1, check out the HSP90AB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSP90AB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSP90 Monoclonal Antibody (M01692-3)
Hello CJ!
M01692-3 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Youwen Zhang,Junye Wang,Shucheng Ye et al.Co-overexpression of STIP1 and Hsp90 correlates with progression and prognosis of lung adenocarcinoma,29 January 2020,PREPRINT (Version 2) available at Research Square [https://doi.org/10.21203/rs.2.13680/v2]
Species: Human
M01692-3 usage in article: APP:IHC, SAMPLE:LAC TISSUE, DILUTION:1:300
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-HSP90 Monoclonal Antibody
Question
See below the WB image, lot number and protocol we used for pancreas using anti-HSP90 Monoclonal antibody M01692-3. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-14
Question
Our lab want to know about to test anti-HSP90 Monoclonal antibody M01692-3 on human pancreas for research purposes, then I may be interested in using anti-HSP90 Monoclonal antibody M01692-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-09
Answer
The products we sell, including anti-HSP90 Monoclonal antibody M01692-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-09
Question
Does anti-HSP90 Monoclonal antibody M01692-3 work for IHC with pancreas?
B. Collins
Verified customer
Asked: 2017-12-12
Answer
According to the expression profile of pancreas, HSP90AB1 is highly expressed in pancreas. So, it is likely that anti-HSP90 Monoclonal antibody M01692-3 will work for IHC with pancreas.
Boster Scientific Support
Answered: 2017-12-12
Question
Will anti-HSP90 Monoclonal antibody M01692-3 work on monkey ELISA with embryonic kidney?
O. Lewis
Verified customer
Asked: 2017-03-02
Answer
Our lab technicians have not validated anti-HSP90 Monoclonal antibody M01692-3 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-HSP90 Monoclonal antibody M01692-3 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey embryonic kidney in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-03-02
