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Product Info Summary
| SKU: | M33909 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-F, ICC, WB |
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Product info
Product Name
Anti-Hu CD165 Purified Monoclonal Antibody
SKU/Catalog Number
M33909
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD165 Purified Monoclonal Antibody (Catalog# M33909). Tested in Flow Cytometry, IP, WB, IHC-F, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD165 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M33909)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
SN2
Isotype
Mouse IgG1 kappa
Immunogen
CD165 purified from human Molt-4 cell line. The mouse monoclonal antibody SN2, also known as SN2 N6-D11, recognizes an extracellular epitope of CD165, an approximately 37-42 kDa transmembrane glycoprotein expressed mainly on leukemic T cells, double positive and double negative thymocytes (CD4-CD8-, CD4+CD8+), and platelets.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Cross-reactivity with LARGE2 was not determined.
Reactive Species
M33909 is reactive to ABRAXAS1 in Human
Applications
M33909 is guaranteed for Flow Cytometry, IP, IHC-F, ICC, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
46.663kDa
Background of Abraxas
CD165 is a poorly characterized transmembrane protein highly expressed on platelets and many leukemic T cell lines. At lower level it is expressed on a proportion of circulating T cells and monocytes, on thymic epithelium, fibroblasts, epidermal keratinocytes, pancreatic islet cells, and some neurons. It might have a role in adhesion between thymocytes and thymic epithelial cells and it can be used as a marker for tumor progression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (frozen sections): Application note: acetone fixation.
Flow cytometry: Recommended dilution: 1-4 µg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions.
Validation Images & Assay Conditions
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Anti-Hu CD165 Purified (M33909) works in WB application under non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, Molt-4, Raji, and Hep-G2 cell lines, mixed and heated (100°C, 5 min) with non-reducing SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel. Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody M33909 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed. CD165 molecules were detected at ~34-48 kDa in Jurkat and Molt-4 cell lines under non-reducing conditions.
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Separation of Jurkat cells stained using anti-human CD165 (M33909) purified antibody (concentration in sample 0,6 μg/ml, GAM APC, red) from Jurkat cells unstained by primary antibody (GAM APC, black) in flow cytometry analysis (surface staining).
Protein Target Info & Infographic
Gene/Protein Information For ABRAXAS1 (Source: Uniprot.org, NCBI)
Gene Name
ABRAXAS1
Full Name
BRCA1-A complex subunit Abraxas 1
Weight
46.663kDa
Superfamily
FAM175 family
Alternative Names
BRCA1-A complex subunit Abraxas; ABRA1; CCDC98; family with sequence similarity 175, member A ABRAXAS1 ABRA1, CCDC98, FAM175A abraxas 1, BRCA1 A complex subunit BRCA1-A complex subunit Abraxas 1|abraxas protein|coiled-coil domain containing 98|family with sequence similarity 175 member A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ABRAXAS1, check out the ABRAXAS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ABRAXAS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hu CD165 Purified Monoclonal Antibody (M33909)
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No publications found for M33909
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