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Product Info Summary
| SKU: | M05830 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, ICC, WB |
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Product info
Product Name
Anti-Hu CD229 Purified LY9 Monoclonal Antibody
SKU/Catalog Number
M05830
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD229 Purified LY9 Monoclonal Antibody (Catalog# M05830). Tested in Flow Cytometry, IP, WB, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD229 Purified LY9 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05830)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
HLy9.25
Isotype
Mouse IgG1
Immunogen
CD299-transfected 300.19 pre-B cell line. The mouse monoclonal antibody HLy9.25 (also known as HLy9.1.25) recognizes an extracellular epitope of CD229 / Ly9, a 100-120 kDa cell surface glycoprotein expressed on T and B cells.
Cross-reactivity
This antibody weakly cross-reacts with murine SIT.
Reactive Species
M05830 is reactive to LY9 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
72.1 kDa
Background of LY9
CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05830 is guaranteed for Flow Cytometry, IP, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 6 μg/ml; positive control: peripheral blood lymphocytes.
Validation Images & Assay Conditions
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Separation of human CD229 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD229 (HLy9.25) purified antibody (concentration in sample 16 µg/ml) GAM APC.
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Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD229 (HLy9.25) purified antibody (concentration in sample 16 µg/ml) GAM APC.
Specific Publications For Anti-Hu CD229 Purified LY9 Monoclonal Antibody (M05830)
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