Anti-Hu CD229 Purified LY9 Monoclonal Antibody

Product Name

Anti-Hu CD229 Purified LY9 Monoclonal Antibody

View all CD229/SLAMF3/Lymphocyte Antigen 9 Antibodies

SKU/Catalog Number

M05830

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD229 Purified LY9 Monoclonal Antibody (Catalog# M05830). Tested in Flow Cytometry, IP, WB, ICC application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD229 Purified LY9 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05830)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

HLy9.25

Isotype

Mouse IgG1

Immunogen

CD299-transfected 300.19 pre-B cell line. The mouse monoclonal antibody HLy9.25 (also known as HLy9.1.25) recognizes an extracellular epitope of CD229 / Ly9, a 100-120 kDa cell surface glycoprotein expressed on T and B cells.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody weakly cross-reacts with murine SIT.

Reactive Species

M05830 is reactive to LY9 in Human

Applications

M05830 is guaranteed for Flow Cytometry, IP, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

72.139kDa

Background of CD229/SLAMF3/Lymphocyte Antigen 9

CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 6 μg/ml; positive control: peripheral blood lymphocytes.

Validation Images & Assay Conditions

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Figure 1. Flow Cytometry validation of LY9 using Anti-Hu CD229 Purified LY9 Monoclonal Antibody (M05830).

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD229 (HLy9.25) purified antibody (concentration in sample 16 μg/ml) GAM APC.

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Figure 2. Flow Cytometry validation of LY9 using Anti-Hu CD229 Purified LY9 Monoclonal Antibody (M05830).

Separation of human CD229 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD229 (HLy9.25) purified antibody (concentration in sample 16 μg/ml) GAM APC.

Gene/Protein Information For LY9 (Source: Uniprot.org, NCBI)

Alternative Names

CD229 antigen; CD229; cell-surface molecule Ly-9; hly9; Ly9; lymphocyte antigen 9Cell surface molecule Ly-9; mLY9; SLAMF3; T-lymphocyte surface antigen Ly-9 LY9 CD229, SLAMF3, hly9, mLY9 lymphocyte antigen 9 T-lymphocyte surface antigen Ly-9|SLAM family member 3|cell surface molecule Ly-9|signaling lymphocytic activation molecule 3

For more info on LY9, check out the LY9 Infographic

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In this infographic, you will see the following information for LY9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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